二甲双胍对脂多糖致炎性反应小鼠的抗炎作用  

Anti-inflammatory effect of metformin on LPS-induced inflammation in mice

作　　者：刘超 高慧婕 杨强强 杨方浩 孔妮 王宝堃 张腾元 LIU Chao;GAO Hui-jie;YANG Qiang-qiang;YANG Fang-hao;KONG Ni;WANG Bao-kun;ZHANG Teng-yuan(Department of Basic Medical Sciences,School of Pharmacy,Jining Medical University,Rizhao 276826,China;College of Biological Science,Jining Medical University,Rizhao 276826,China)

机构地区：[1]济宁医学院药学院基础医学教研室,山东日照276826 [2]济宁医学院生物科学学院,山东日照276826

出　　处：《基础医学与临床》2019年第9期1248-1251,共4页Basic and Clinical Medicine

基　　金：山东省自然科学基金 （ZR2016HL22）;济宁医学院博士科研基金（JY2015BS23）;山东省 医药卫生科技发展计划（2015WS0420，2017WS338）

摘　　要：目的探究二甲双胍(Met)对脂多糖(LPS)致炎性小鼠的抗炎效应。方法ICR小鼠分为对照组、LPS组、二甲双胍高、中和低剂量组(400,200和100mg/kg)。对照组和LPS组腹腔注射0.9%氯化钠溶液,其余3组注射不同剂量的二甲双胍;0.5h后,除对照组外,其余4组腹腔注射等体积的LPS致小鼠炎性反应,6h后测量小鼠的脾指数;ELISA检测小鼠血清中炎性因子IL-6、TNF-α和IL-10的表达;RT-PCR检测小鼠肝脏组织IL-6、TNF-α和IL-10mRNA表达的变化;HE染色检测小鼠肝脏组织炎性反应变化。结果相对于LPS组,二甲双胍用药组小鼠的脾指数降低(P<0.05);外周血IL-6和TNF-α及肝脏组织中IL-6和TNF-αm RNA的表达明显下降(P<0.05);而外周血IL-10及肝脏组织中IL-10mRNA显示上升(P<0.05);二甲双胍高剂量组可明显减轻由LPS所致小鼠肝脏的炎性反应。结论二甲双胍对于LPS致炎性小鼠具有一定的抗炎活性。Objective To study the anti-inflammatory effect of metformin(Met)on lipopolysaccharide(LPS)-induced inflammation in mice.Methods The mice were divided into control group,LPS group,metformin high dose group(400 mg/kg),metformin medium dose group(200 mg/kg)and metformin low dose group(100 mg/kg).The control group and LPS group were intraperitoneally injected with normal saline,the other groups were injected with different doses of metformin.After 0.5 hours,the control group was injected with normal saline,the other four groups were injected intraperitoneally with LPS.After 6 hours,the spleen index of the mice was measured.The expression of IL-6,TNF-αand IL-10 in mouse serum was detected by ELISA and the expressions of IL-6,TNF-αand IL-10 mRNA in mouse liver were detected by RT-PCR.The inflammatory changes of liver tissues in each group were observed by HE staining techniques.Results Compared with the LPS group,the spleen index,IL-6 and TNF-αexpression in serum and IL-6 and TNF-αmRNA expression in liver were lower in metformin group,however,IL-10 expression in serum and IL-10 mRNA expression in liver were higher in metformin group.The application of metformin(400 mg/kg)reduced the inflammatory changes of liver tissues in mouse with LPS induced inflammation.Conclusions Metformin has certain anti-inflammatory activity against inflammation induced by LPS in mice.

关 键 词：二甲双胍 炎性反应 脂多糖 炎性因子 

分 类 号：R967[医药卫生—药理学]