双氢青蒿素对肺纤维化大鼠ERK和CTGF表达的影响  

Effect of dihydroartemisinin on expression of CTGF and ERK of rats with pulmonary fibrosis

作　　者：王保兰 王立新[1] 朱蓉[1] WANG Bao-lan;WANG Li-xin;ZHU Rong(Department of Respiratory Medicine,Affiliated Huai an No.1 People's Hospital of Nanjing Medical University,Huai an 223000,Jiangsu,China)

机构地区：[1]南京医科大学附属淮安第一医院呼吸科

出　　处：《川北医学院学报》2019年第4期338-342,共5页Journal of North Sichuan Medical College

基　　金：江苏省卫生计生委医学科研课题立项(H201554)

摘　　要：目的:探讨双氢青蒿素对博来霉素诱导肺纤维化大鼠肺组织中Col1(a2)、CTGF、ERK基因及蛋白表达的影响。方法: 75只SD大鼠随机分成假手术对照组、模型对照组、溶剂对照组、阳性对照组、和双氢青蒿素治疗组5组,每组各15只;每组再按处死时间点分为7 d、14 d、28 d三个亚组,每个亚组5只。除假手术对照组外,所有动物气管内注入博来霉素5 mg/kg。造模后第一天起,相应予生理盐水、生理盐水、5%羧甲基纤维素钠、泼尼松5 mg/kg、双氢青蒿素75 mg/kg,1 次/d,持续至处死当天。结果:大鼠肺纤维化模型构建成功,双氢青蒿素治疗组大鼠肺组织中CTGF、ERK1/2、pERK1/2蛋白及ERK1、ERK2、Col1(a2)基因的表达量低于模型对照组( P <0.01)。结论:双氢青蒿素可抑制肺纤维化大鼠肺组织ERK和CTGF的表达,减轻实验大鼠的肺纤维化程度。Objective: To explore the effects of dihydroartemisinin on the expression of Col1(a2),CTGF and ERK of rats with pulmonary fibrosis induced by bleomycin. Methods: 75 SD rats were randomly divided into 5 groups:sham-operated control group,model control group,solvent control group,positive control group and dihydroartemisinin treatment group,15 cases in each group.Each group was divided into three subgroups according to the time point of execution:7 d,14 d,28 d.With the exception of sham-operated control group,all animals received intratracheal injection of bleomycin 5 mg/kg.Normal saline,5% carboxymethyl cellulose sodium,prednisone 5 mg/kg,dihydroartemisinin 75 mg/kg were administered once a day until the day of death. Results: The rat model of pulmonary fibrosis was successfully constructed.The expression levels of CTGF,ERK1/2,pERK1/2 protein and ERK1,ERK2,Col1 (a2) gene in the lung tissue of the dihydroartemisinin treated group were lower than those of the model control group ( P <0.01). Conclusion: Dihydroartemisinin can inhibit the expression of ERK and CTGF in lung tissue of rats with pulmonary fibrosis,and reduce the degree of pulmonary fibrosis in experimental rats.

关 键 词：双氢青蒿素 肺纤维化 CTGF ERK 

分 类 号：R285.5[医药卫生—中药学]