黄牛SH2B1基因多态性及其与体尺性状的关联性分析  

Association of SNPs in SH2B1 Gene with Growth Traits in Yellow Cattle

作　　者：张毅威 桂林生[1] 郭文莉 胡言 余横伟 杨武才[1] 昝林森[1] 赵春平[1] ZHANG Yi-wei;GUI Lin-sheng;GUO Wen-li;HU Yan;YU Heng-wei;YANG Wu-cai;ZAN Lin-sen;ZHAO Chun-ping(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100)

机构地区：[1]西北农林科技大学动物科技学院

出　　处：《中国牛业科学》2019年第4期1-6,共6页China Cattle Science

基　　金：陕西省重点研发计划项目(2018ZDXM-NY-046);杨凌示范区协同创新重大项目(2017CXY-14);国家重点研发计划项目(2018YFD0501700);杨凌示范区科技计划项目(2016NY-03);国家肉牛牦牛产业技术体系项目(CARS-37)

摘　　要：为探讨SH2B1基因在黄牛中的组织表达规律和遗传多态性,采集3头2周岁秦川母牛的6种组织,构建SH2B1基因的组织表达谱;此外,以4个中国地方黄牛品种为研究对象,包括秦川牛、郏县红牛、巴山牛和南阳牛,结合DNA测序技术检测SH2B1基因的多态位点,并将其与秦川牛生长发育的指标进行了关联分析。qPCR结果表明,SH2B1基因在秦川牛组织中呈泛表达状态,并在背最长肌组织中表达最高。DNA测序分析在SH2B1基因的第4内含子和3′UTR共发现了2个单核苷酸多态位点,分别命名为C6073T和T8067C。关联性分析结果表明,C6073T能够显著影响体长、坐骨端宽和胸围,而T8067C能能够显著影响体长和体高。综上所述,笔者推测SH2B1基因可以作为影响秦川牛生产性能的遗传标记候选基因。To investigate the expression pattern and genetic polymorphism of SH2B1 gene in yellow cattle, six kinds of tissues were collected from three 2-year-old Qinchuan cattle. Then RNAs were extracted and qPCR was conducted after transcription to obtain the expression pattern in these six tissues. Additionally, DNAs were extracted from four local yellow cattle, Qinchuan, Jiaxian, Bashan, and Nanyang cattle. PCR combined with DNA sequencing to detect the polymorphisms of SH2B1 gene and analyzed the genetic diversity. Later, the association of different genotypes of SNPs in SH2B1 with growth traits in Qinchuan cattle was studied. qPCR results showed that SH2B1 gene generally expressed in these six kinds of tissues. DNA sequencing identified two SNPs in the fourth intron and 3′ UTR, namely C6073 T and T8067 C. Association study results showed that C6073 T can significantly affect body length, ischial end width and chest circumference, while T8067 C can significantly affect body length and height. To summarize, SH2B1 gene could be a candidate gene used for production traits in Qinchuan cattle.

关 键 词：SH2B1基因 表达模式 SNP 体尺性状 

分 类 号：S823.8[农业科学—畜牧学] Q347[农业科学—畜牧兽医]