滇中牛Y-SNPs和Y-STRs遗传多样性研究  

Genetic Diversity of Y-SNPs and Y-STRs in Dianzhong Cattle

作　　者：贾鹏 王凯悦 亐开兴[2] 张继才[2] 黄必志[2] 苏相生 陈宏 雷初朝[1] JIA Peng;WANG Kai-yue;QU Kai-xing;ZHANG Ji-cai;HUANG Bi-zhi;SU Xiang-sheng;CHEN Hong;LEI Chu-zhao(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100;Yunnan Academy of Grassland and Animal Science,Kunming 650212;Shuangbai Center for Animal Disease Control and Prevention,Shuangbai,Yunnan 675100)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]云南省草地动物科学研究院,昆明650212 [3]云南省双柏县动物疫病预防控制中心,云南双柏675100

出　　处：《中国牛业科学》2019年第4期7-9,62,共4页China Cattle Science

基　　金：国家肉牛牦牛产业技术体系项目(CARS-37);云南省后备人才培养计划项目(2018HB045);重点研发项目(2018BB028)

摘　　要：[目的]为探究云南滇中牛的Y染色体遗传结构与血统来源,以期为该黄牛品种的资源保护与利用提供科学依据。[方法]采用PCR扩增和荧光分型方法,对27头滇中牛的Y-SNPs(UTY19和ZFY10)和Y-STRs(INRA189和BM861)遗传多样性进行分析。[结果]27头滇中牛包含Y2和Y3两种单倍型组,其频率分别为22.22%和77.78%。结合Y-SNPs和Y-STRs的分型结果,发现这27头滇中牛存在Y2-90-158、Y3-88-156和Y3-90-156共3种Y染色体单倍型(Y-SNP-INRA189-BM861),Y染色体单倍型多样度为0.5128±0.0904,其遗传多样性较高。[结论]滇中牛Y染色体遗传多样性较高,有普通牛和瘤牛2个父系起源,以瘤牛血统为主。[Objective] We explored the genetic structure and paternal origins of Y chromosome in Yunnan Dianzhong cattle to provide a scientific basis for the resource protection and utilization.[Methods] PCR amplification and fluorescence genotyping methods were used to detect the genetic diversity of Y-SNPs(UTY19 and ZFY10)and Y-STRs(INRA189 and BM861)in 27 Dianzhong cattle samples.[Results] All 27 Dianzhong samples belong to Y2 and Y3 haplogroup with frequencies of 22.22% and 77.78%, respectively. The Y chromosome haplotypes of Y2-90-158, Y3-88-156 and Y3-90-156 were identified in 27 Dianzhong cattle samples by combining Y-SNPs and Y-STRs genotyping data(Y-SNP-INRA189-BM861). The Y chromosome haplotype diversity was 0.5128±0.0904 indicating high paternal genetic diversity in Dianzhong cattle.[Conclusions] Dianzhong cattle has high paternal genetic diversity and two paternal origins of Bos taurus Y2 and Bos indicus Y3, of which, Bos indicus Y3 is obviously predominant paternal origin.

关 键 词：滇中牛 Y-SNPS Y-STRS 遗传多样性 父系起源 

分 类 号：S823.81[农业科学—畜牧学] S823.94[农业科学—畜牧兽医]