清喉咽合剂高效液相指纹图谱研究  

Study on HPLC Fingerprint of Qinghouyan Mixture

作　　者：姜范成 梁云 鄢长余 赵月然 孙睿 刘莹 JIANG Fan-cheng;LIANG Yun;YAN Zhang-yu;ZHAO Yue-ran;SUN Rui;LIU Ying(Dalian Inspection and Certification Technology Service Center, Dalian Liaoning 116021,China;Dalian Food and Drug Safety Complaint Reporting and Acceptance Center, Dalian Liaoning 116021,China)

机构地区：[1]大连市检验检测认证技术服务中心,辽宁大连116021 [2]大连市食品药品安全投诉举报受理中心,辽宁大连116021

出　　处：《中国药物评价》2019年第4期271-274,共4页Chinese Journal of Drug Evaluation

摘　　要：目的:建立清喉咽合剂高效液相色谱指纹图谱分析方法,为其科学鉴定和质量控制提供依据。方法:色谱柱为ZORBAXSB-C18(4.6mm×250mm,5μm);流动相为乙腈-3%醋酸水溶液梯度洗脱;流速1.0mL·min^-1;检测波长270nm;柱温30℃。结果:清喉咽合剂色谱峰得到了较好的分离,建立了清喉咽合剂指纹图谱共有模式,确定了19个共有指纹峰、7个特征峰及所有色谱峰的分布情况。结论:该方法简便、重复性好、可操作性强,可用于建立清喉咽合剂指纹图谱质量标准和清喉咽合剂质量控制。Objective : To establish a high performance liquid chromatographic fingerprint analysis method for Qinghouyan mixture, and provide evidence for its scientific identification and quality control. Methods:The column was ZORBAX SB-C 18 (4.6 mm×250 mm, 5 μm). The mobile phase was gradient elution with acetonitrile-3% acetic acid solution. The flow rate was 1.0 mL·min^-1 . The detection wavelength was 270 nm. The column temperature was 30 ℃. Results:The chromatographic peaks of Qinghouyan mixture were well separated. The common pattern of fingerprints of Qinghouyan mixture was established. The distribution of 19 common fingerprint peaks, 7 characteristic peaks and all chromatographic peaks were determined. Conclusion :The method is simple, reproducible and operability, and can be used to establish the quality standard of Qinghouyan mixture and the quality control of Qinghouyan mixture.

关 键 词：清喉咽合剂 高效液相色谱 指纹图谱 质量控制 

分 类 号：R927.11[医药卫生—药学]