竞争免疫荧光分析法高灵敏、高选择性检测赭曲霉毒素  

Sensitive and Selective Detection of Ochratoxin a Using Fluorescent Competitive Immunoassay

作　　者：任姿静 张迎红 吕鑫 张远馥 王术皓[1] REN Zi-jing;ZHANG Ying-hong;LV Xin;ZHANG Yuan-fu;WANG Shu-hao(School of Chemistry and Chemistry Engineering,Liaocheng University,Liaocheng 252059,China)

机构地区：[1]聊城大学化学化工学院

出　　处：《聊城大学学报（自然科学版）》2019年第6期66-70,共5页Journal of Liaocheng University:Natural Science Edition

基　　金：国家自然科学基金项目（21705066,21675072）;山东省自然科学基金项目（ZR2013BQ016）资助

摘　　要：基于半抗原与二抗竞争结合固相抗体的免疫模式,建立了一种高灵敏、高选择性检测赭曲霉毒素的新型免疫荧光分析法.赭曲霉毒素具有半抗原性,可与基底抗体作用.当赭曲霉毒素加入到该体系后,与异硫氰酸荧光素标记的羊抗兔(IgG-FITC)发生竞争反应,随着赭曲霉毒素浓度的增加,IgG-FITC与抗体结合的浓度减少,荧光强度降低.在最佳实验条件下,荧光强度的变化值(ΔF])与赭曲霉毒素浓度的对数在1nmol/L-100μmol/L范围内呈良好的线性关系,相关系数R=0.9932.方法的检出限为0.35nmol/L.将该方法用于不同种类的酒样中赭曲霉毒素的测定,结果令人满意.与传统方法相比,本方法是一种低成本、高灵敏度、高选择性的检测方法,在食品安全领域中具有潜在的应用价值.A highly sensitive and selective competitive immunoassay method is presented for fluorescence determination of ochratoxin A (OTA) based on competitive immune reaction between OTA and fluorescein isothiocyanate-labeled goat anti-rabbit (IgG-FITC).In the work,the fluorescence of IgG-FITC decreased with the increasing of the concentration of OTA,since IgG-FITC was replaced by OTA.The assay showed a good linear toward OTA concentration in the range of 1 nmol/L to 100 μmol/L with a correlation of 0.9932.The limit of detection for OTA was experimentally determined to be 0.34 nM.The sensing system has been used for the determination of OTA in drink samples.Relative to conventional methods,this method offers the advantages of high-throughput,sensitive,low cost and selectivity,showing great potential for food security.

关 键 词：荧光分析 免疫竞争反应 赭曲霉毒素 

分 类 号：O657[理学—分析化学]