壬基酚暴露对SD大鼠心肌组织的影响研究  

Effects of nonylphenol exposure on myocardial tissue of SD rats

作　　者：杨誉佳[1] 刘超 陈乃华 许洁[2] YANG Yujia;LIU Chao;CHEN Naihua;XU Jie(Department of Gynecology,Affiliated Hospital of Zunyi Medical College,Zunyi,Guizhou 563000,China;School of Public Health,Zunyi Medical College,Zunyi,Guizhou 563000,China)

机构地区：[1]遵义医学院附属医院妇科,贵州遵义563000 [2]遵义医学院公共卫生学院,贵州遵义563000

出　　处：《现代医药卫生》2019年第17期2593-2595,2599,共4页Journal of Modern Medicine & Health

基　　金：国家自然科学基金项目（81560527,81760580）;贵州省科技厅基金重点项目（黔科合基础2018-1429）;贵州省教育厅拔尖人才基金项目（黔教合KY2018054）;贵州省科学技术厅优秀青年科技人才资助项目（黔科合平台人才〔2017〕5612）;贵州省卫生健康委员会科学技术基金项目（gzwjkj2016-1-045,gzwjkj2017-1-053）

摘　　要：目的探讨壬基酚暴露对SD大鼠心肌组织的影响。方法选取健康雌性SD大鼠24只,按体重随机分为4组,每组6只。实验组分别给予25、50、100mg/kg壬基酚灌胃染毒,对照组给予植物油灌胃,每天1次,共42d。计算各组SD大鼠心脏脏器系数,采用分光光度法测定心肌组织Na^+K^+-腺嘌呤核苷三磷酸(ATP)酶、Ca^2+Mg^2+-ATP酶的活性,通过苏木精-伊红染色(HE染色)观察心肌组织病理变化。结果实验组SD大鼠体重、Na^+-K^+-ATP酶活性与对照组比较,差异均无统计学意义(P>0.05)。与对照组比较,实验组SD大鼠心脏质量、心脏系数、Ca^2+Mg^2+-ATP酶活性显著下降,差异均有统计学意义(P<0.05),且心脏质量、心脏系数与染毒剂量呈负相关(P<0.05)。病理组织学结果显示,对照组心肌组织纤维排列致密,结构清晰。25mg/kg染毒组未见病理形态改变,50mg/kg染毒组可见间质中有少量炎症细胞浸润,100mg/kg染毒组可见纤维排列比较疏松,部分心肌纤维结构破坏,细胞核消失,间质中炎症细胞浸润情况也较50mg/kg染毒组显著。结论壬基酚暴露可导致心肌组织炎性反应,使Ca^2+Mg^2+-ATP酶活性下降。ObjectiveTo investigate the effects of nonylphenol exposure on myocardial tissue in SD rats.MethodsTwenty-four healthy female SD rats were randomly divided into 4 groups according to body weight,with 6 rats in each group.The experimental group was given 25,50,100 mg/kg nonylphenol by intragastric administration,and the control group was given vegetable oil by gavage once a day for 42 days.The cardiac organ coefficients of SD rats in each group were calculated.The activity of Na^+K^+-adenosine triphosphate(ATP)ase and Ca^2+Mg^2+-ATPase in myocardial tissue was determined by spectrophotometry.Pathological changes of myocardial tissue were observed by hematoxylin-eosin staining(HE staining).ResultsCompared with the control group,there was no significant difference in body weight,Na^+-K^+-ATPase activity of the SD rats in the experimental group(P>0.05).Compared with the control group,the heart mass,cardiac coefficient,Ca^2+Mg^2+-ATPase activity of the SD rats in the experimental group decreased significantly,and the differences were statistically significant(P<0.05).Cardiac mass and cardiac coefficient of SD rats in the experimental group were negatively correlated with the dose(P<0.05).Histopathological results showed that the myocardial fibers of the control group were densely arranged and the structure was clear.No pathological changes were observed in the 25 mg/kg group.In the 50 mg/kg group,a small amount of inflammatory cells infiltrated in the interstitium.In the 100 mg/kg group,the fiber arrangement was loose,some myocardial fiber structure was destroyed,the nucleus disappeared,and the inflammatory cell infiltration in the interstitial was also significantly higher than that in the 50 mg/kg group.ConclusionExposure to nonylphenol can cause an inflammatory response in myocardial tissue,and make Ca^2+Mg^2+-ATPase activity decrease.

关 键 词：壬基酚 大鼠心肌组织 炎症细胞 Na^+K^+-ATP酶 Ca^2+Mg^2+-ATP酶 

分 类 号：R114[医药卫生—卫生毒理学]