EB病毒新近感染患者IgG类抗体实验室检测分析  

Laboratory assays and analysis of IgG subclass antibodies in patients recently infected by epstein barr virus

作　　者：陈礼文[1] 程娟[1] 王秀 梁伟[1] 管世鹤[1] CHEN Li-Wen;CHENG Juan;WANG Xiu;LIANG Wei;GUAN Shi-He(Clinical Laboratory,the Second Hospital of Anhui Medical University,Hefei 230601,China)

机构地区：[1]安徽医科大学第二附属医院检验科

出　　处：《中国免疫学杂志》2019年第17期2122-2125,共4页Chinese Journal of Immunology

基　　金：安徽省自然科学基金项目(1808085MH229)

摘　　要：目的:研究EB病毒(EBV)衣壳抗原(VCA)-IgM+新近感染患者抗核抗原(NA)-IgG、VCA-IgG和抗早期抗原(EA)-IgG表达特点。方法:微粒子化学发光免疫法检测EBV抗体,选择160例VCA-IgM+新近感染患者统计分析VCA-IgG、NA-IgG和EA-IgG阳性例数(百分率);将VCA-IgM+/NA-IgG-界定为原发感染的早期/急性期,VCA-IgM+/NA-IgG+界定为原发感染恢复期或再感染,或以年龄(7岁)分组,比较分析二类感染不同阶段患者以及<7岁和≥7岁患者VCA-IgG、EA-IgG或NA-IgG实验室检测结果及其临床意义。结果:160例VCA-IgM+患者NA-IgG、VCA-IgG和EA-IgG阳性例数分别为50例(31.25%)、91例(56.88%)和16例(10%),全阴性60例(37.5%),全阳性9例(5.63%)。EBV原发感染早期/急性期和原发感染恢复期或再感染患者EA-IgG浓度(中位数)分别为0.80(四分位间距0.50~1.35)AU/ml和0.76(四分位间距0.54~1.61)AU/ml(P=0.546),阳性率分别为6.36%和18%(P=0.023);VCA-IgG浓度(中位数)分别为1.27(四分位间距0.32~3.90)AU/ml和22.93(四分位间距7.26-50)AU/ml(P=0.000),阳性率分别为40.91%和92%(P=0.000)。<7岁(126例)和≥7岁(34例)患者的NA-IgG阳性例数分别为30例(23.81%)和20例(58.82%)(P=0.000);VCA-IgG阳性例数分别为70例(55.56%)和21例(61.76%)(P=0.517);EA-IgG阳性例数分别为13例(10.32%)和3例(8.82%)(P=1.000)。结论:EBV新近感染患者IgG类抗体分布特征明显,联合检测EBV抗体有助于EB新近感染的实验室确诊以及感染时相判断。Objective: To investigate the expression features of VCA-IgG,NA-IgG and EA-IgG in VCA-IgM + patients recently infected by epstein barr virus (EBV). Methods: Serum levels of EBV antibodies,including VCA-IgG,NA-IgG,EA-IgG and VCA-IgM,were detected by chemiluminescence microparticle immunoassay (CMIA).160 VCA-IgM + patients were included in the study and the numbers and percentages of positive specimens of VCA-IgG,NA-IgG or EA-IgG were calculated respectively.VCA-IgM +/NA-IgG -and VCA-IgM +/NA-IgG + were characterized as early/acute stage of primary infection and recovery stage or reinfection respectively,and the IgG subclass antibodies were compared between the two groups.Age-related differential expression of VCA-IgG,NA-IgG and EA-IgG in VCA-IgM + patients were also analyzed. Results: The numbers and percentages of positive specimens of NA-IgG,VCA-IgG and EA-IgG in 160 VCA-IgM + patients were 50 (31.25%),91 (56.88%) and 16 (10%),and the uniform positive and negative samples of the three IgG antibodies were 9 (5.63%) and 60 (37.5%),respectively.The median concentration of EA-IgG in VCA-IgM +/NA-IgG -and VCA-IgM +/NA-IgG + were 0.80 AU/ml with a range 0.50-1.35 AU/ml and 0.76 AU/ml with a range 0.54-1.61 AU/ml ( P = 0.546),and the positive rates of EA-IgG in the two groups were 6.36% and 18%( P =0.023),respectively.Similarly,the concentration and positive rates of VCA-IgG in the two groups were 1.27 AU/ml with a range 0.32-3.90 AU/ml and 22.93 AU/ml with a range 7.26-50 AU/ml ( P =0.000),40.91% and 92%( P =0.000),respectively.Furthermore,the positive samples and rates of NA-IgG,VCA-IgG and EA-IgG between <7 ( n =126) and ≥7 ( n =34) were 30 (23.81%) and 20 (58.82%)( P =0.000),70 (55.56%) and 21 (61.76%)( P =0.517),and 13 (10.32%) and 3 (8.82%)( P =1.000),respectively. Conclusion: The distribution of IgG subclass antibodies in patients recently infected by EBV is of distinctive characteristics.Combined detection of EBV antibodies contributes to the laboratory diagnosis and phase discrimination of recent EBV infecti

关 键 词：疱疹病毒 原发感染 新近感染 微粒子化学发光免疫测定 抗体 

分 类 号：R446.6[医药卫生—诊断学]