2种杓兰属植物内生狭截盘多毛孢菌的分离与鉴定  

Isolation and Identification of Endophytic Truncatella angustata in Roots of Two Cypripedium Species

作　　者：徐玲玲[1] 赵桂仿[2] 孙敬祖[3] 张焱[1] 陶贵荣[1] XU Lingling;ZHAO Guifang;SUN Jingzu;ZHANG Yan;TAO Guirong(Key Laboratory of Plant-microbe Collaboration Research, School of Biological and Environmental Engineering, Xi an University , Xi an 710065, China;Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, School of Life Sciences, Northwest University, Xi an 710069, China;State Key Laboratory of Mycology , Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China)

机构地区：[1]西安文理学院生物与环境工程学院,植物微生物协同研究重点实验室,西安710065 [2]西北大学生命科学学院,西部资源生物与现代生物技术教育部重点实验室,西安710069 [3]中国科学院微生物研究所真菌学国家重点实验室,北京100101

出　　处：《西北植物学报》2019年第7期1316-1321,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31300366);西安市科技计划项目(CXY1531WL18,2017CGWL02)

摘　　要：该文报道了西藏杓兰(Cypripediumtibeticum)和无苞杓兰(C.bardolphianum)根中内生真菌新记录种——狭截盘多毛孢(Truncatella angustata)。该研究从四川黄龙沟不同海拔区分别采集到西藏杓兰和无苞杓兰,并在其根中分离获得148个狭截盘多毛孢菌株。形态学观察显示,狭截盘多毛孢菌株在PDA培养基上菌落白色,背面黄褐色;分生孢子器黑色脓包状;分生孢子梭型,4个细胞,3个隔膜;顶端附属丝1~5根。ITSrDNA分析发现3种新基因型菌株(HLIO15_15a_22、HLIO15_20a_42和HLIO15_17a_46);在海拔最高(3330~3400m)的居群中狭截盘多毛孢的分离率最高,且包含全部3种基因型菌株。构建截盘多毛孢属(Truncatella)真菌ITS序列系统发育树,发现狭截盘多毛孢不同生态功能的菌株没有形成明显分支。One new record of endophytic fungus, Truncatella angustata , in roots of Cypripedium tibeticum and C. bardolphianum , was reported in this paper. 148 strains of Truncatella angustata were isolated from the roots of C. tibeticum and C. bardolphianum as endopytes at different altitudinal areas in Huanglong Valley, Sichuan Province, China. Morphological descriptions of T. angustata revealed that the colony on PDA pale yellow, reverse of the culture yellowish brown;pycnidia black, pustule-like;conidia fusiform, 4-celled, 3-septate;apical appendages 1-5. Three genotypes (HLIO15_15a_22, HLIO15_20a_42 and HLIO15_17a_46) were identified by ITS rDNA analysis. The isolation rate of T. angustata was highest in the highest altitude population, containing all three genotype strains. The phylogenetic tree of the ITS sequence of the genus Truncatella was constructed. It was found that the strains with different ecological functions of the genus Truncatella did not form obvious clades.

关 键 词：狭截盘多毛孢 兰科植物 形态 生态功能 

分 类 号：Q948.12[生物学—植物学] Q938.1