基于亚铁离子催化分光光度法测定葡萄糖  

The determination of the Glucose based on Copper Ion Catalytic Spectrophotometry

作　　者：马业菲 付小芬 于丽平 何文 高燕 褚然然 Ma Yefei;Fu Xiaofen;Yu Liping;He Wen;Gao Yan;Chu Ranran(Qilu Instituteof Technology,Jinan 250000,China)

机构地区：[1]齐鲁理工学院

出　　处：《山东化工》2019年第16期122-123,126,共3页Shandong Chemical Industry

摘　　要：Fe(Ⅱ)能够催化过氧化氢(H2O2)快速氧化3,3',5,5'-四甲基联苯胺(TMB)从而引起吸光度的变化。葡萄糖氧化酶(GOD)分解葡萄糖产生H2O2,通过测定H2O2的含量可间接测葡萄糖的含量。实验考察并优化了反应时间,pH值和温度等影响参数。在最佳实验条件下(pH值5.5,TMB浓度2.0×10^-3mol/L,Fe(Ⅱ)浓度2.0×10^-4mol/L,H2O2浓度2.0×10^-4mol/L,反应时间30min,温度37℃),葡萄糖浓度在2.0×10^-3~1.0×10^-2mol/L范围内与吸光度具有良好的线性关系,线性方程为:I=0.1064C+0.2248,且相关系数R^2=0.9983。将该方法用于检测糖尿病患者的尿液,实现了对实际样品的分析和检测,具有一定的实际意义。Fe(Ⅱ) can catalyze hydrogen peroxide(H2O2) rapid oxidation of the 3,3’,5,5’-tetramethylbenzidine(TMB) and produce absorbance change.Glucose oxidase decomposes glucose to produce hydrogen peroxide.The content of glucose can be determined indirectly by measuring the content of hydrogen peroxide.Under the optimal conditions(pH 5.5,TMB 2.0×10^-3mol/L,Fe(Ⅱ) 2.0×10^-4mol/L,H2O2 2.0×10^-4mol/L,reaction time 30 min and the temperature 37℃),there is a linear relationship between the concentration of glucose and absorbance can be obtained over the range of 2.0×10^-3 to 1.0×10^-2 mol/L.The linear equation was: I = 0.1064 C + 0.2248,the correlation coefficient was R^2=0.9983.The method was used to detect urine of diabetic patients and realizes the analysis and detection of actual samples,which had certain practical significance.

关 键 词：葡萄糖 Fe(Ⅱ) 分光光度法 

分 类 号：O657.32[理学—分析化学]