盐酸小檗碱对高脂喂养大鼠棕色脂肪组织PGC-1α及UCP-1基因表达的影响  

Effect of Berberine Hydrochloride on PGC-1Α/UCP-1 Gene Expression in Brown Adipose Tissue of Rats Fed with High-Fat Diet

作　　者：喻日成[1] 范元硕[1] 罗建华[1] YU Richeng;FAN Yuanshuo;LUO Jianhua(Department of Endocrinology, Guizhou Provincial People′s Hospital, Guiyang, Guizhou 550002, China)

机构地区：[1]贵州省人民医院内分泌科

出　　处：《贵州医科大学学报》2019年第8期908-911,共4页Journal of Guizhou Medical University

基　　金：贵州省人民医院国家自然科学基金培育基金[黔科合平台人才(2018)5764-05]

摘　　要：目的:探讨盐酸小檗碱对高脂喂养的SD大鼠棕色脂肪组织解偶联蛋白-1(UCP-1)、过氧化物酶体增殖物激活受体γ辅助激活因子(PGC-1α)基因表达的影响。方法:6周龄SD雄性大鼠24只,随机分为对照组、高脂喂养组及盐酸小檗碱组,高脂喂养组及盐酸小檗碱组大鼠给与高脂饮食,第6周时盐酸小檗碱组大鼠灌胃盐酸小檗碱,对照组和高脂喂养组大鼠灌喂等体积生理盐水;实验第24周时,取大鼠肩胛部位棕色脂肪组织行HE染色观察各组大鼠组织学变化,应用Real-TimeRT-PCR及Westernblot检测棕色脂肪组织UCP-1、PGC-1αmRNA及蛋白表达。结果:对照组大鼠棕色脂肪组织HE染色可见脂肪细胞体积小、大小均匀,而高脂喂养组脂肪细胞体积大、不均匀、细胞间有融合,盐酸小檗碱组棕色脂肪细胞体积较高脂组明显缩小、细胞分布均匀;高脂喂养组大鼠肩胛棕色脂肪组织UCP-1、PGC-1αmRNA及蛋白表达水平较对照组下降,而盐酸小檗碱组较高脂喂养组水平上调。结论:盐酸小檗碱改善肥胖大鼠的胰岛素抵抗与上调大鼠棕色脂肪组织UCP-1、PGC-1α的基因表达有关。Objective: To investigate the effects of berberine hydrochloride on PGC-1α/UCP-1 gene expression in brown adipose tissue(BAT)of rats fed with high-fat diet. Methods: 24 male Sprague-Dawley (SD) rats (6 weeks) were divided into 3 groups including control group,high fat diet group (HFD group) and berberine hydrochloride group (BH group).The two experiment groups were both fed with high fat diet,BH group was given berberine hydrochloride,control group and HFD group were given saline water equal to subject's volume.At 24 weeks,scapular brown adipose tissue was stained with HE to observe histological changes of rats' tissues of all groups;the expressions of UCP-1,PGC-1α mRNA genes and protein were analyzed by Real-Time RT-PCR and Western blot. Results: HE staining of brown adipose tissue from control group showed small adipocyte with uniform in size,while adipocytes were big and non-uniform in size with cell fusion in HFD groups;brown adipocyte from BH group was significantly smaller than that of HFD group with equally distributed cells.Expressions of UCP-1,PGC-1α mRNA genes and protein from brown adipocyte cells of HFD group was lower than control group,while BH group showed up-regulated expressions of UCP-1,PGC-1α mRNA genes and protein than HFD group. Conclusion: These results suggest that berberine hydrochloride remarkably improved obesity rats' insulin resistance and enhanced expressions of UCP-1,PGC-1α mRNA genes of brown adipocyte tissue of high fat diet fed rats.

关 键 词：盐酸小檗碱 解偶联蛋白-1 过氧化物酶体增殖物激活受体γ辅助激活因子 棕色脂肪组织 高脂喂养 大鼠 Sprague-Dawley 

分 类 号：R589.2[医药卫生—内分泌]