肾脏移植早期T细胞受体Vβ链CDR3克隆谱系分析  

Diversity analysis of T cell receptor Vβ CDR3 in early stage of kidney transplantation

作　　者：张聪 李兴库 于雅迪 金艾顺[1] ZHANG Cong;LI Xingku;YU Yadi;JIN Aishun(College of Basic Medicine,Harbin Medical University,Harbin 150086,China)

机构地区：[1]哈尔滨医科大学基础医学院

出　　处：《免疫学杂志》2019年第9期805-810,共6页Immunological Journal

摘　　要：目的探讨肾脏移植患者早期(第1个月内)外周血单个核细胞(PBMC)来源的T细胞受体Vβ链CDR3区(TCR Vβ CDR3)克隆扩增分析。方法收集10对肾脏移植受者及供者外周血分离PBMC并提取DNA。使用HLA-ABDR配型试剂盒检测其HLA-ABDR分型,分析配型情况。收集肾脏移植患者移植前和移植后早期PBMC,并提取RNA后逆转录成cDNA,使用TCR Vβ CDR3特异性引物,扩增TCR并测序和分析克隆扩增情况。最后,对克隆扩增的TCR Vβ CDR3片段序列进行比较分析。结果检测和比对了10对肾脏移植受体和供体的HLA-ABDR分型。移植患者移植前未发现TCR克隆扩增现象;而与此相比,移植后早期不同时间(第7、14、28天)均发现单克隆或寡克隆扩增。克隆扩增的TCR Vβ CDR3氨基酸基因片段部分序列有重复出现现象。结论肾脏移植患者早期外周血TCR Vβ CDR3出现克隆扩增可能与移植相关。本研究为今后肾脏移植配型以及免疫排斥反应的防治提供参考。To investigate the clonal expansion of T cell receptor (TCR) Vβ CDR3 which were considered as immune responses after kidney transplantation,we collected peripheral blood mononuclear cells (PBMC) from 10 pairs of kidney transplant recipients and donors,and detected HAL-ABDR matching by HLA-ABDR matching kit. The TCR Vβ CDR3 genes were amplified in samples of PBMC at different time points before and after kidney transplantation using specific primers.The PCR products were further labeled with fluorescent and analyzed by genescan technique for the CDR3 size,to evaluate clonality of the detectable TCR Vβ T cells.Genescan analysis showed that clonal expansion of TCR Vβ T cells could be found in the early period after kidney transplantation,and some sequences of amino acids were shared in clonal expansion of T cells.In conclusion,the clonal expansion of TCR Vβ CDR3 in patients with kidney transplantation may be related to transplantation.This study provides a research basis for future kidney transplantation matching and prevention of immune rejection.

关 键 词：HLA表型 T细胞受体 肾脏移植早期 互补决定区 克隆扩增 

分 类 号：R392.4[医药卫生—免疫学]