慢性宫内缺氧子代主动脉差异microRNA表达谱研究  

Study on differential microRNA expression profiles in the aorta of chronic intrauterine hypoxic offsprings

作　　者：李雅雅 吕国荣[1,2] 林惠通 陈娇虹[1] 朱燕云 LI Ya-Ya;LYU Guo-Rong;LIN Hui-Tong(Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China)

机构地区：[1]福建医科大学附属第二医院超声医学科,福建泉州362000 [2]泉州医学高等专科学校省级母婴健康服务技术协同创新中心,福建泉州362100 [3]厦门市儿童医院超声医学科,福建厦门361006

出　　处：《中国妇幼保健》2019年第16期3809-3812,共4页Maternal and Child Health Care of China

基　　金：福建省卫生和计划生育委员会项目(2015-CX-27)

摘　　要：目的研究慢性宫内缺氧对子代主动脉microRNA (miRNA)表达谱的影响.方法将16只金黄地鼠孕鼠随机分为宫内缺氧组(CIH)和正常对照组(NC),取4周龄子代主动脉进行数字化基因表达谱测序,并进行qRT-PCR验证.结果与NC组相比,CIH组共有9个miRNA上调,10个miRNA下调,靶基因涉及多种代谢酶及炎症因子;KEGG Pathway分析显示差异miRNA在多个炎症及氧化应激相关信号通路富集明显.结论 CIH可诱导子代主动脉miRNA表达谱发生改变,并可能通过差异miRNA参与其子代成年期心血管疾病的发生发展.Objective To research the effect of chronic intrauterine hypoxia ( CIH) on offspring aorta microRNA ( miRNA) expression profile. Methods Sixteen pregnant golden hamsters were randomly divided into CIH group and normal control ( NC) group. Differentially expressed miRNA of aorta in 4-week-old offsprings were examined and identified by RT-PCT. Results Compared with NC group, 9 miRNAs were upregulated and 10 miRNAs were down-regulated in CIH group, the target genes involved multiple metabolic enzymes and inflammatory factors. KEGG Pathway analysis showed that differential miRNAs were significantly enriched in multiple inflammatory pathways and oxidative stress signaling pathways. Conclusion CIH leads to the changes of miRNA expression profile in aorta of offsprings, which may be the possible mechanism of CIH related cardiovascular diseases in adulthood of offsprings .

关 键 词：慢性宫内缺氧 心血管疾病 微小核糖核酸 数字化基因表达谱 

分 类 号：R714.3[医药卫生—妇产科学]