青藏高原土著柴油降解菌群的筛选及其鉴别  

Isolation and Identification of Indigenous Diesel-degrading Bacteria in the Qinghai-Tibetan Plateau

作　　者：董志龙[1] 高慧娟 吕沛诚 王宝山[1] DONG Zhi-long;GAO Hui-juan;L Pei-cheng;WANG Bao-shan(School of Environment and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;School of Science,Hangzhou Normal University,Hangzhou 310012,China)

机构地区：[1]兰州交通大学环境与市政工程学院,兰州730070 [2]杭州师范大学理学院,杭州310012

出　　处：《兰州交通大学学报》2019年第4期97-104,共8页Journal of Lanzhou Jiaotong University

基　　金：国家自然科学基金(51368029);甘肃省重点研发计划(17YF1FA114)

摘　　要：为实现青藏高原低温柴油降解菌群的筛选及鉴别,采用人工模拟污染、实验室筛选及高通量测序方法,研究了柴油土著微生物的降解特性及功能降解菌群.结果表明:青藏高原地区土壤温度季节性差异明显,夏季温度较高,10℃成为柴油降解土著微生物筛选的最佳温度;柴油污染土样与未污染土样微生物数量存在差异,前者微生物丰度最高;环境温度10℃下,第17d柴油降解率达62.63%,8个菌属具有石油烃类物质降解能力,总占比52.50%,其中高效嗜油菌是柴油降解主体.研究成果可供多年冻土区石油污染生物修复提供参考.To achieve the isolation and identification of low temperature-resistant diesel-degrading Bacteria in soils from the Qinghai-Tibetan Plateau,the research means of artificial simulation of pollution,laboratory isolation and high-throughput sequencing were employed to study the degradation characteristics of indigenous microorganisms and bacteria with the function of biodegradation.The results showed that the seasonal differences of soil temperature were marked,and the higher temperature occurred in the summer.As a consequence,10℃became the optimal temperature of the isolation of indigenous microorganisms with diesel-degrading.The differences existed in microbial numbers of uncontaminated(surface,middle)soil samples and diesel-contaminated soil samples with the highest microbial abundance.Under the environmental condition of 10℃,The degradation efficiency of diesel on the 17th day was 62.63%.The 8 genera were identified with degradation function of petroleum hydrocarbon pollutants,totally accounting for 52.50%,among which high efficiency diesel-degraded bacteria were the principal part of biodegradation.The research results provide a reference for the bioremediation of oil pollution in permafrost regions.

关 键 词：青藏高原 高通量测序 土著微生物 柴油污染 生物降解 

分 类 号：X53[环境科学与工程—环境工程]