特殊离子对嗜酸性铁氧化混合菌活性的影响研究  

Effect of Special ions on Activity of Acidophilic Ferric Oxide Mixtures

作　　者：刘顺亮 李泽兵[1] 史维浚[1] 蒋亚飞[1] 张晓玲 孙占学[1] Liu Shunliang;Li Zebing;Shi Weijun;Jiang Yafei;Zhang Xiaoling;Sun Zhanxue(State Key Laboratory cf Nuclear Resources and Environment, East China University of Technology, Nanchang 330031, China)

机构地区：[1]东华理工大学核资源与环境省部共建国家重点实验室

出　　处：《稀有金属》2019年第8期854-862,共9页Chinese Journal of Rare Metals

基　　金：国家自然科学基金项目(41772266,51564001,51408112);江西省重点研发计划项目(20161BBH80051);科技部国际合作项目(2011DFR60830);江西省教育厅科技计划项目(GJJ14495);核资源与环境国家重点实验室培育基地开放基金项目(NRE1407);东华理工大学博士启动金项目(DHBK2013207),东华理工大学研究生创新资金项目(YC2017-S272)资助

摘　　要：为了探明铀矿浸出过程中不同氧化剂处理所得的吸附尾液对浸矿液制备体系中嗜酸性铁氧化混合菌的影响机制,本研究利用实际浸矿尾液作为培养基,以Acidithibacillus ferrooxidans,Leptospirillum ferrooxidans及Acidithibacillus thiooxidans等为主的3种混合菌种作为受试材料,通过批示实验研究含不同浓度Cl^-, Al^3+和Mn^2+的浸铀尾液体系对混合菌生长和铁氧化活性的影响。结果表明:混合菌在各离子类型尾液中的生长呈现为二段式生长,且抑制效应主要表现于延滞期阶段,其中Mn^2+和Al^3+所导致的抑制属于多代次培养后可适应的影响因素,延滞期依次:0~300 h (Cl^-), 10~30 h (Al^3+)和10~30 h (Mn^2+);各离子对混合菌铁氧化活性的影响差异较大,其抑制率由大到小的顺序为Cl^->Al^3+>Mn^2+;高含量的离子抑制混合菌的生物铁氧化能力, 741 mmol·L^-1的Al^3+或1092 mmol·L^-1的Mn2+或192 mmol·L^-1的Cl^-能完全抑制混合菌的生长。研究结果将为推动铁氧化细菌在浸矿尾液再生体系内的稳定应用进程提供理论指导和实验支持。In order to find out the influence mechanism of adsorption tails processed by different oxidants in the leaching process of uranium ore on the mixed bacteria of eosinophilic iron oxidein the preparation system of leaching ore, the actual leaching tail fluid was used as the culture mediumin this study, with mainly Acidithibacillus ferrooxidans, Leptospirillum ferrooxidans and Acidithibacillus thiooxidans these three mixed bacteria as tested material, and the effects of leaching uranium tail fluid system with different concentrations of Al^3+, Mn^2+ and Cl^- on the growth of mixed bacteria were investigated. The results showed that the growth of the mixed bacteria in the tail of each ion type was a two-stage growth, and the inhibitory effect was mainly manifested in the lag phase. Among the inhibitory effect, the inhibition caused by Mn^2+ and Al^3+ belonged to adaptable factors in multiple generations. After incubation, the lag time was 0 to 300 h(Cl^-), 10 to 30 h(Al^3+), and 10 to 30 h(Mn^2+), respectively. The inhibition effect of different ions on the iron oxidizing activity of mixed bacteria differed greatly, and the descending order was Cl^->Al^3+>Mn^2+. High content of ions inhibited the biological iron oxidation capacity of mixed bacteria. The growth of mixed bacteria on the Al^3+, Mn^2+or Cl^- threatening condition(741, 1092 or 192 mmol·L^-1) was completely suppressed. The research results provided theoretical guidance and experimental support for promoting the stable application process of iron-oxidizing bacteria in leaching tail fluid regeneration systems.

关 键 词：离子胁迫效应 浸铀尾液 混合菌群 生长动力学 

分 类 号：TF18[冶金工程—冶金物理化学]