白英生物碱对人宫颈癌HeLa细胞的抑制作用  

Inhibitory effect of Solanumlyratum Thunberg alkaloids on human cervical cancer HeLa cells

作　　者：丁书军[1] 廖洁 张琛晓 黄丽霞[1] 黄宁[3] 汤旭[1] 余兴火 DING Shu-jun;LIAO Jie;ZHANG Chen-xiao;HUANG Li-xia;HUANG Ning;TANG Xu;YU Xing-huo(Department of Clinical Laboratory, Jiangxi Maternal and Child Health Hospital, Nanchang 330006, China;Department of Clinical Laboratory, People′s Hospital of Pingxiang City, Jiangxi Province, Pingxiang 337000, China;Prenatal Diagnosis Center, Jiangxi Maternal and Child Health Hospital, Nanchang 330006, China)

机构地区：[1]江西省妇幼保健院检验科,江西南昌330006 [2]江西省萍乡市人民医院检验科,江西萍乡337000 [3]江西省妇幼保健院产前诊断中心,江西南昌330006

出　　处：《中国当代医药》2019年第24期34-36,共3页China Modern Medicine

基　　金：江西省卫生计生委中医药科研课题(2017B080)

摘　　要：目的探讨白英生物碱(STA)对人宫颈癌HeLa细胞的抑制作用。方法提取STA并制备4.0、8.0、16.0mg/L等3种浓度的STA反应体系,分别定义为反应体系A、B、C,设置空白对照,培养24h后通过MTT法检测各反应体系光密度(OD)值和对人宫颈癌HeLa细胞增殖的抑制效果。结果不同浓度STA反应体系的OD值比较,差异均有统计学意义(P<0.05);反应体系C的OD值显著低于空白对照、反应体系A及B,差异均有统计学意义(P<0.05);反应体系B的OD值显著低于空白对照和反应体系A,差异均有统计学意义(P<0.05);反应体系A的OD值显著低于空白对照,差异有统计学意义(P<0.05)。不同浓度STA反应体系的细胞生长抑制率比较,差异均有统计学意义(P<0.05);反应体系C的细胞生长抑制率显著高于反应体系A和B,差异均有统计学意义(P<0.05);反应体系B的细胞生长抑制率显著高于反应体系A,差异有统计学意义(P<0.05)。结论STA对人宫颈癌HeLa细胞增殖有一定的抑制作用。Objective To investigate the inhibitory effect of solanumlyratum Thunberg alkaloid (STA) on human cervical cancer HeLa cells. Methods Extraction of STA and preparation of three concentration STA reaction systems 4.0, 8.0, 16.0 mg/L were defined as reaction systems A, B and C, respectively, and the blank control was set. The optical density (OD) value of each reaction system and the inhibitory effect on the proliferation of human cervical cancer HeLa cells were detected by MTT method after 24 hours of culture. Results The OD values of STA reaction systems at different concentrations were significantly different (P<0.05). The OD value of reaction system C was significantly lower than that of blank control, reaction system A and B, the differences were statistically significant (P<0.05). OD value of reaction system B was significantly lower than that of blank control and reaction system A, the differences were statistically significant (P<0.05). OD value of reaction system A was significantly lower than that of blank control, the difference was statistically significant (P<0.05). The growth inhibition rates of STA reaction system at different concentrations were significantly different (P<0.05). The growth inhibition rate of reaction system C was significantly higher than that of reaction system A and B, the differences were statistically significant (P<0.05). The growth inhibition rate of reaction system B was significantly higher than that of reaction system A, the difference was statistically significant (P<0.05). Conclusion STA can inhibit the proliferation of human cervical cancer HeLa cells.

关 键 词：白英生物碱 人宫颈癌HELA细胞 细胞增殖 

分 类 号：R711.74[医药卫生—妇产科学]