输尿管热损伤后TGF-β亚型及α-SMA表达的实验研究  

Experimental study of transforming growth factor β subtype and α smooth muscle actin expression after ureteral thermal injury

作　　者：刘昕[1] 孙毅海[1] 唐深[2] 陶卫琦[1] 李少红[1] 黄才胜[1] 黄超斌 黄慧宁 谢阳 LIU Xin;SUN Yihai;TANG Shen;TAO Weiqi;LI Shaohong;HUANG Caisheng;HUANG Chaobin;HUANG Huining;XIE Yang(Department of Urology,the Second People′s Hospital of Nanning,Nanning 530031,China;School of Preclinical Medicine,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西南宁市第二人民医院泌尿外科,南宁市530031 [2]广西医科大学基础医学院,南宁市530021

出　　处：《微创医学》2019年第4期412-414,429,共4页Journal of Minimally Invasive Medicine

基　　金：南宁市科技局资助项目(编号:201530)

摘　　要：目的探讨输尿管钬激光损伤后瘢痕形成相关因子(TGF-β亚型及α-SMA)的表达情况。方法采用定量反转录-聚合酶链反应(RT-PCR)技术测定正常兔输尿管以及钬激光损伤后兔输尿管TGF-β亚型(TGF-β1、TGF-β2、TGF-β3)及α-SMA的表达水平,并进行统计学分析。结果与正常兔输尿管比较,钬激光损伤兔输尿管3周后TGF-β1、TGF-β2及α-SMA表达量明显增高(P<0.05),而两组TGF-β3表达差异无统计学意义(P>0.05)。结论钬激光输尿管热损伤导致TGF-β亚型及α-SMA表达发生变化,与输尿管瘢痕形成及狭窄发生密切相关。Objective To investigate the expression of scar formation-related factors[transforming growth factorβ(TGF-β)subtype andαsmooth muscle actin(α-SMA)]after holmium laser-caused ureteral injury.Methods Quantitative reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the expression levels of TGF-βsubtypes(TGF-β1,TGF-β2,TGF-β3)andα-SMA in normal rabbit ureter and injured rabbit ureter caused by holmium laser,and the data were statistically analyzed.Results Compared with normal rabbit ureter,injured rabbit ureter caused by holmium laser exhibited notably elevated expression of TGF-β1,TGF-β2 andα-SMA after 3 weeks of injury(P<0.05),but no statistically significant difference was found in TGF-β3 expression between the two groups(P>0.05).Conclusion Holmium laser-caused ureteral thermal injury results in changes in the expression of TGF-βsubtype andα-SMA,which is closely related to the formation of ureter scar and the occurrence of ureter stricture.

关 键 词：输尿管 钬激光 损伤 TGF-β 亚型 Α-SMA 

分 类 号：R693.5[医药卫生—泌尿科学]