Apelin-13在血液透析内瘘狭窄患者中的表达及意义  

Expression and significance of Apelin-13 in hemodialysis patients with fistula stenosis

作　　者：顾雪梅[1] 刘磊[1] GU Xuemei;LIU Lei(Department of Nephropathy,TCM Hospital of Mianyang City,Sicuang,Mianyang 621000,China)

机构地区：[1]四川省绵阳市中医医院肾病科

出　　处：《河北医药》2019年第18期2725-2729,共5页Hebei Medical Journal

摘　　要：目的探讨血管紧张素Ⅰ受体样受体(APJ)的内源性配体13(APJ endogenous ligand 13,apelin-13)在血液透析内瘘狭窄患者中的表达意义。方法选取血液透析内瘘狭窄患者和非血液透析内瘘狭窄患者分别作为试验组和对照组,每组30例。用酶联免疫吸附试验和一氧化氮检测试剂盒检测2组患者血清中apelin-13、血管紧张素Ⅱ(AngⅡ)、一氧化氮(NO)的浓度。在细胞水平,用apelin-13干扰序列和无义序列转染人脐静脉内皮细胞HUVECs,以及用AngⅡ、apelin-13刺激HUVECs,然后通过Western blot的方法检测细胞中磷酸化内皮型一氧化氮合酶(peNOS)和非磷酸化内皮型一氧化氮合酶(endothelial NO synthase,eNOS)的表达情况,通过一氧化氮检测试剂盒检测细胞培养上清中NO的含量。结果内瘘狭窄患者血清中apelin-13[(52.94±5.46)pg/ml]和NO[(54.87±5.48)μm/ml]均低于非内瘘狭窄患者apelin-13[(80.66±9.63)pg/ml]和NO[(72.23±8.20)μm/ml],差异有统计学意义( P <0.05)。而内瘘狭窄组血清AngⅡ浓度[(172.57±11.75)pg/ml]高于非内瘘狭窄组AngⅡ水平[(146.43±7.56)pg/ml],差异有统计学意义( P <0.05)。在细胞水平上发现,相比无义序列转染的细胞,apelin-13 shRNA转染的细胞产生的peNOS和NO减少,差异有统计学意义( P <0.01)。当用AngⅡ刺激细胞时,发现AngⅡ刺激的细胞产生的peNOS和NO显著低于未经AngⅡ刺激组,但当用AngⅡ与apelin-13共刺激细胞,细胞产生的peNOS和NO表达恢复。结论Apelin-13与AngⅡ相互拮抗作用,共同介导信号通路eNOS/NO调控血管狭窄,适当增加apelin-13的浓度可促进信号通路eNOS/NO,缓解血管狭窄。Objective To investigate the expression and significance of endogenous ligand 13(Apelin-13) of angiotensin receptor-like 1(APJ) in hemodialysis patients with fistula stenosis.Methods Thirty patients with internal fistula stenosis who received hemodialysis in our hospital were enrolled as experimental group,and the other 30 patients with internal fistula stenosis who did not receive hemodialysis were enrolled as control group.The levels of Apelin-13,angiotensin Ⅱ(AngⅡ),nitric oxide(NO) were measured by enzyme-linked immunosorbent assay(ELISA) and nitric oxide detection kit.At the cellular level,human umbilical vein endothelial cells(HUVECs) were transfected with Apelin-13 interference sequence and nonsense sequence,and were stimulated with AngⅡ and Apelin-13.Moreover the levels of phosphorylated endothelial nitric oxide synthase(peNOS) and nonphosphorylated endothelial nitric oxide synthase(eNOS) were detected by Western Blot,and the expression levels of NO were detected by nitric oxide detection kit.Results The serum levels of Apelin-13 [(52.94±5.46)pg/ml] and NO [(54.87±5.48)μm/ml] in patients with internal fistula stenosis were significantly lower than those of Apelin-13 [(80.66±9.63)pg/ml] and NO [(72.23±8.20)μm/ml] in patients without internal fistula stenosis.However the serum levels of AngⅡ[(172.57±11.75)pg/ml] in patients with internal fistula stenosis were significantly higher than those [(146.43±7.56)pg/ml] in patients without internal fistula stenosis(P<0.05).At the cellular level,the production of peNOS and NO in the cells transfected with Apelin-13 shRNA was lower than that in the cells transfected with nonsense sequence(P<0.01).When AngⅡ was used to stimulate cells,it was found that the production of peNOS and NO by AngⅡ-stimulated cells was significantly lower than that of non-AngⅡ-stimulated cells,however, if both AngⅡ and apelin-13 were used to stimulate cells,the expression of peNOS and NO was recovered.Conclusion Apelin-13 has antagonistic action with AngⅡ each other,th

关 键 词：自体动静脉内瘘 APELIN-13 血管紧张素Ⅱ 一氧化氮 人脐静脉内皮细胞 护理 

分 类 号：R459.5[医药卫生—治疗学]