肺泡灌洗液结核杆菌RNA和DNA检测在痰菌阴性肺结核诊断中的临床应用  

Clinical application of tuberculosis RNA and DNA detection in alveolar lavage fluid for diagnosis of sputum negative pulmonary tuberculosis

作　　者：陈皋[1] 邬碧波[1] 吴孟征 罗万蓉[1] 秦春俊 刘舒舒[1] 刘建英[1] 刘丽[1] CHEN Gao;WU Bi-bo;WU Meng-zheng;LUO Wan-rong;QIN Chun-jun;LIU Shu-shu;LIU Jian-ying;LIU Li(Department of Infectious Diseases,Deyang People’s Hospital,618000,China)

机构地区：[1]德阳市人民医院感染科

出　　处：《传染病信息》2019年第4期344-345,共2页Infectious Disease Information

摘　　要：目的观察肺泡灌洗液结核杆菌RNA和DNA检测在痰菌阴性肺结核患者中的阳性检出情况。方法选取2017年3月—2019年3月在我院接受检查和治疗且最终确诊为痰菌阴性肺结核的患者为研究对象,所有患者均接受肺泡灌洗液结核杆菌RNA和DNA检测。分析2种检测方法阳性检出率的差异。结果结核杆菌RNA阳性检出率为63.33%,DNA阳性检出率为33.33%,前者高于后者。结论结核杆菌RNA检测对痰菌阴性肺结核具较高灵敏度,优于DNA检测。Objective To observe the positive detection of tuberculosis RNA and DNA in alveolar lavage fluid in sputum negative patients with pulmonary tuberculosis.Methods From March 2017 to March 2019,patients with sputum negative pulmonary tuberculosis who were examined,diagnosed and treated in our hospital were selected as the subjects.All patients were tested for tuberculosis RNA and DNA in alveolar lavage fluid.The difference of positive detection rate between the 2 methods was analyzed.Results The positive detection rate of tuberculosis RNA and DNA was 63.33%and 33.33%respectively.The former was higher than the latter.Conclusions Tuberculosis RNA detection is highly sensitive to sputum negative pulmonary tuberculosis than DNA detection.

关 键 词：痰菌阴性 肺结核 核酸检测 灵敏度 

分 类 号：R521[医药卫生—内科学]