高脂饮食诱导青春期前雌性大鼠多囊卵巢综合征的实验研究  

High fat diet exposure induced polycystic ovarian syndrome in prepuberty rats

作　　者：古兰·托合提木拉提 叶尔努尔·吐苏甫汗[2] 马玉兰 玛依努尔·尼亚孜[1] Gulan Tuohetimulati;Yemur Tusuvhan;Yulan Ma;Mnyinuer Niyaz(Xinjiang Autonomous Region People’s Hospital, Urumqi, Xinjiang 830001, China;The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830028, China)

机构地区：[1]新疆自治区人民医院,新疆乌鲁木齐830001 [2]新疆医科大学第二附属医院,新疆乌鲁木齐830028

出　　处：《中国现代医学杂志》2019年第18期13-19,共7页China Journal of Modern Medicine

摘　　要：目的复制高脂饮食诱导青春期前雌性大鼠的多囊卵巢综合征(PCOS)动物模型,探讨其生殖和代谢特征。方法将青春期前雌性大鼠随机分成C组和D组,C组接受正常饮食,D组接受高脂饮食,连续喂养105d。另将成年雌性大鼠随机分成A组和B组,A组接受1%羧甲基纤维素,B组接受1mg/kg灌服来曲唑,连续喂养21d。观察各组体重、动情周期和卵巢组织学变化,检测各组空腹血糖、胰岛素、糖耐量、计算胰岛素抵抗指数(HOMA-IR)、定量胰岛素敏感性检测指数、血脂水平、性激素水平、促黄体激素受体(LHR)及促卵泡激素受体(FSHR)mRNA的表达。结果B组0、7、14及21d体重低于A组(P<0.05);B组观察结束后卵巢重量高于A组(P<0.05);B组喂养21d后糖耐量曲线下面积(AUC)高于A组(P<0.05);D组49、77及105d的糖耐量AUC、空腹胰岛素、HOMA-IR、血清总胆固醇、雌二醇及孕酮高于C组(P<0.05),C组定量胰岛素敏感性检测指数、睾酮高于D组(P<0.05)。结论高脂饮食可诱导青春期前雌性大鼠PCOS,并产生代谢紊乱和卵巢改变,与PCOS患者临床观察相似。Objective To establish a polycystic ovary syndrome (PCOS) animal model of prepubertal female rats induced by high-fat diet, and to explore the reproductive and metabolic characteristics of PCOS rats. Methods Prepubertal female rats were randomly divided into two groups: the control group which received a normal diet (group C, n = 9), and the experimental group which received a high-fat diet (group D, n = 9), and both two groups had been fed for 105 days. In addition, the adult female rats were randomly divided into another two groups: the control group which received 1% carboxymethyl cellulose (group A, n = 9), and the other group which received 1 mg/kg of letrozole (group B, n = 9), and both groups had been fed for 21 days. The body weight, estrus cycle and histology of ovary in the four groups were observed. Fasting glucose, fasting insulin, glucose tolerance, homeostasis model assessment for insulin resistance (HOMA-IR) and quantitative insulin sensitivity check index (QUICKI), lipid profile, hormonal profile, leutinizing hormone receptor (LHR) and follicle stimulating hormone receptor (FSHR) mRNA expression were also measured. Results The body weight and ovarian weight of group B were lighter than that of group A (P < 0.05). Compared to group A, the AUC of glucose tolerance increased (P < 0.05). The AUC of glucose tolerance, fasting insulin, HOMA-IR, serum total cholesterol, estradiol and progesterone of 49, 77 and 105 days in group D were higher than those in group C (P < 0.05), and the quantitative insulin sensitivity index and testosterone in group C were higher than those in group D (P < 0.05). Conclusions Prepuberty highfat diets induce PCOS in female rats and produce metabolic disorders and ovarian changes that are similar to those observed in patients with PCOS.

关 键 词：多囊卵巢综合征 大鼠 模型 动物 

分 类 号：R711.75[医药卫生—妇产科学]