Aerolysin单分子界面的构建及高选择性单分子检测  

Construction and High Selectivity of Aerolysin Single Molecular Interface for Single Molecule Analysis

作　　者：武雪原 应佚伦[1,2] 龙亿涛 WU Xueyuan;YING Yilun;LONG Yitao(School of Chemistry and Molecular Engineering,East China University of Science and Technology, Shanghai 200237,China;State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering, Nanjing University,Nanjing 210023,China)

机构地区：[1]华东理工大学化学与分子工程学院,上海200237 [2]南京大学化学化工学院,生命分析化学重点实验室,南京210023

出　　处：《高等学校化学学报》2019年第9期1825-1831,共7页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21834001,61871183)资助~~

摘　　要：提出了一种基于Aerolysin膜蛋白质分子构建单分子界面的方法,运用蛋白质工程技术对单分子界面进行定点修饰,所建立方法灵活、可控且重复性好.采用Poly(dA)4为探针分子对修饰后的单分子界面进行了表征,结果表明,在孔口处的Arginine修饰影响了寡聚核苷酸的选择性.为进一步理解Aerolysin单分子界面及合理设计功能性单分子界面提供了参考.As an new conceptual single molecular interface, the biomolecular nanopore can accommodate only a single molecule and contains abundant amino acid residues in the lumen, providing an appropriate confinement for single molecule detection. Herein, a generalized method is proposed for the construct of single molecular interface based on membrane protein. The method is flexible, controllable and repeatable. The characterization of D209R mutation with Poly(dA) 4 illustrate that the selectivity of aerolysin single molecular interface is related the electrostatic properties at the entrance of nanopore, which deepens our understanding of aerolysin single molecular interface, providing a paradigm for rational design of functional single molecular interface.

关 键 词：气单胞菌溶素 单分子界面 纳米孔道 单分子分析 

分 类 号：O657[理学—分析化学]