基于DNA条形码分子技术的海龙及其混伪品鉴定研究  

Identification of Syngnathus and Its Adulterants Using DNA Barcoding

作　　者：张红印[1] 金平 刘冬[1] 高晓晨[1] 张辉[1] ZHANG Hong-yin;JIN Ping;LIU Dong;GAO Xiao-chen;ZHANG Hui(Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学

出　　处：《中国现代中药》2019年第9期1181-1185,共5页Modern Chinese Medicine

基　　金：公益性行业科研专项(201507002)

摘　　要：目的:基于DNA条形码分子技术对动物药海龙及其混伪品进行鉴别,建立一种统一的海龙鉴定技术手段。方法:对收集到的动物药海龙及其混伪品进行DNA提取、PCR扩增和双向测序检测,运用CodonCodeAligner进行序列拼接,采用MEGA7.0软件进行序列比对分析,比较种内、种间序列差异,构建动物药海龙及其混伪品的系统发育树。结果:所有海龙药材及其混伪品均可以使用中药材DNA条形码分子鉴定技术进行鉴别,共得到7个物种129条序列,各物种之间序列区分明显。结论:中药材DNA条形码鉴定技术能够准确有效地鉴别动物药海龙及其混伪品,可以作为海龙类中药的统一鉴定方法,为海龙的临床用药安全提供了新的分子技术手段。Objective: To establish a standard method to identify the traditional Chinese medicine of Syngnathus basing on the DNA barcoding. Methods: The Syngnathus and its adulterants were performed on DNA extraction,PCR amplification and bi-directional DNA sequencing,sequence assembly and consensus sequence generation were performed using the CodonCode Aligner.Neighbor-joining tree construction was carried out by MEGA 7.0.The intra-specific variation and the inter-specific divergence were calculated. Results: All the species could be identified based on DNA sequences database of TCM of deer which contained 129 samples of 7 species. Conclusion: DNA barcoding of TCM can accurately and effectively identify Syngnathus and its adulterants.It can be used as a unified identification method of Syngnathus and provide a new molecular technology for the clinical medication safety of TCM.

关 键 词：海龙 中药材DNA条形码鉴定技术 混伪品 

分 类 号：R282.74[医药卫生—中药学] R282.5[医药卫生—中医学]