全蝎DNA条形码分子鉴定研究  

Identification of Scorpion Using DNA barcoding Technique

作　　者：庞中化[1] 王志刚[1] 成小兰[1] 胡春萍[1] 曹鹏[1] PANG Zhong-hua;WANG Zhi-gang;CHENG Xiao-lan;HU Chun-ping;CAO Peng(Jiangsu Province Academy of Traditional Chinese Medicine,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine,Nanjing 210028,China)

机构地区：[1]江苏省中医药研究院/南京中医药大学附属中西医结合医院

出　　处：《中国现代中药》2019年第9期1192-1196,1205,共6页Modern Chinese Medicine

基　　金：公益性行业科研专项(201507002)

摘　　要：目的:本研究通过COI序列对全蝎及其混伪品进行DNA条形码鉴别,为全蝎药材分子鉴定提供科学依据。方法:通过野外采集东亚钳蝎正品,对采集样品形态学鉴定、DNA提取、PCR扩增COI序列,测序建立东亚钳蝎的正品COI数据库,通过比对确定东亚钳蝎正品和伪品的DNA条形码。结果:实验共获取310份东亚钳蝎样本。通过序列分析,确定东亚钳蝎COI序列长度为658 bp。采用MEGA 6.0软件对东亚钳蝎样品及混伪品进行序列比对,构建邻接(NJ)树。结果表明东亚钳蝎COI序列扩增成功,与混伪品COI序列明显区分。结论:本研究运用COI条形码序列可准确鉴定全蝎的基原动物及其混伪品,为后续全蝎药材的分子鉴定提供了新的可行性方法。Objective: DNA barcoding is becoming more and more attractive as a new method for species identifcation.In this paper,we discussed the validation of COI gene sequence to identify Buthus martensii. Methods: Genomic DNA of scorpion were extracted,and the COI regions were PCR amplified and sequenced.The COI database of scorpion was established,the authentic and fake products of scorpion was compared using MEGA 6.0 software. Results: A total of 310 scorpion samples were collected.Through sequence analysis,658 bp length of the COI sequence was determined.Phylogenetic analysis was carried out with different methods,including neighbor joining and bayesian analysis.The results showed that the COI gene sequence of the Buthus martensii was successfully amplified,and could be clearly distinguished from the fake products. Conclusion: DNA Barcoding is efficient and useful for species identification.This study could provide a new method for the molecular identification of scorpion.

关 键 词：全蝎 东亚钳蝎 COI序列 DNA条形码 分子鉴定 

分 类 号：R282.74[医药卫生—中药学] R282.5[医药卫生—中医学]