白扁豆中派可林酸的定性定量研究  

Study on Qualitative and Quantitative methods for Pipecoline Acid of Lablab Semen Album

作　　者：陈丹 李柯[2] 侯茜 吴梅青 李若存[2] CHEN Dan;LI Ke;HOU Xi;WU Meiqing;LI Ruocun(Xiangtan Medical and Health Vocational and Technical College, Xiangtan 411102,China;Hunan Academy of TCM, Changsha 410013,China)

机构地区：[1]湘潭医卫职业技术学院,湖南湘潭411102 [2]湖南省中医药研究院,湖南长沙410013

出　　处：《中国民族民间医药》2019年第16期56-59,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：湘潭市科技局一般项目(ZJ20171027)

摘　　要：目的:建立白扁豆中派可林酸的定性定量研究方法。方法:采用TLC法对派可林酸进行鉴别;采用HPLC建立派可林酸的含量测定方法,采用2,4-二硝基氟苯柱前衍生,色谱柱为Hypersil BDS C18 (4.6 mm×250 mm,5μm),以乙腈-二甲基甲酰胺-0.025 mol/L醋酸钠溶液(21∶0.5∶79)为流动相,检测波长为390 nm,流速为l mL/min,柱温30℃。结果:在TLC色谱中检出派可林酸;派可林酸在4.6~46μg/mL浓度范围内具有良好的线性关系,r=0.9992,加样回收率为97.61%~104.83%,RSD为2.79%。结论:该方法可靠,数据准确,重现性好。Objective To establish qualitative and quantitative methods for Pipecoline acid of Lablab semen album. Methods Pipecoline acid was identified by TLC.The contents of Pipecoline acid was determined by HPLC.The sample was derivatized with chiral compound 2,4-Dinitrofluorobenzene and the separarion was performed on a Hypersil BDS C18 column(4.6 mm×250 mm, 5 μm) by using the mobile phase consisted of CⅡ3 CN-(CⅡ3)2 NCOⅡ-0.025 mol/L CH3 COONa(21∶0.5∶79), the flow rate was 1 mL/min, the detection wavelength was set at 390 nm and the column temperature at 30℃. Results Pipecoline acid can be distinguished and has good separation. The linear range of Pipecoline acid was 4.6~46 μg/mL(r=0.9992). The recoveries ranged from 97.61% to 104.83%, RSD was 2.79%. Conclusion The method is reliable,accurate and reproducible.

关 键 词：白扁豆 定性定量研究 高效液相色谱法 派可林酸 

分 类 号：R284.1[医药卫生—中药学]