肉蔻-5味丸对慢性抑郁模型大鼠血清IL-1和IL-6含量的影响  

Experimental Research on Nutmeg Wuwei Pills Against of Depression Model Rat serum IL-1 and IL-6 Content

作　　者：呼日乐巴根[1] 乌兰其其格[1] 莲花[1] 宏梅 乌日罕[1] 木奇 佟海英[2] HU Rilebagen;WU Lanqiqige;LI Anhua;HONG Mei;WU Rihan;MU Qi;TONG Haiying(Inner Mongolia Medical University,Inner Mongolia Hohhot 010110,China;Beijing University of Chinese Medicine, Beijing 100029,China)

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010110 [2]北京中医药大学,北京100029

出　　处：《中国民族民间医药》2019年第15期9-12,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：内蒙古自然科学基金项目(2015MS0893);内蒙古医科大学创新团队发展计划项目(YKD2017KJCXTD006)

摘　　要：目的:观察肉蔻-5味丸对慢性抑郁模型大鼠血清IL-1和IL-6含量的影响,探讨其抗抑郁机制。方法:60只雄性SD大鼠,随机分为正常组、模型组、西药组(盐酸氟西汀0.0036g/kg),蒙药低、中、高剂量组(分别给予肉蔻-5味丸0.2、0.4、0.8g/kg),每组10只。建立CUMS模型,同时ig给药,每天1次,连续给药21d。以体重变化、糖水消耗实验进行评价模型,ELISA检测大鼠血清IL-1和IL-6的含量。结果:实验过程中模型组大鼠体重增长较缓慢,糖水消耗量降低,应激结束时,与正常组比较模型大鼠血清IL-1和IL-6的含量明显升高,而盐酸氟西汀和蒙药肉蔻-5味丸均可不同程度改善模型大鼠抑郁状态。与模型组比较,给予盐酸氟西汀和蒙药肉蔻-5味丸高、中剂量组大鼠血清IL-1和IL-6含量明显降低(P<0.05);低剂量组大鼠血清IL-1和IL-6含量有降低趋势,但无统计学意义。结论:蒙药肉蔻-5味丸可通过抑制模型大鼠血清IL-1和IL-6含量升高发挥抗抑郁作用。Objective To observe the effect of Mongolia medicine Nutmeg Wuwei Pills on the depressive the expression of IL-1 and IL-6 content of depression rat model and explore the mechanism. Methods Sixty male SD rats were randomly divided into normal group,model group, western medicine group (fluoxetine hydrochloride 0.0036 g/kg) and Mongolia medicine low、medium and high does groups(were given Nutmeg Wuwei pills 0.2、0.4、0.8 g/kg),10 rats in each group and built CUMS model.While administered the medicine by the way of ig once a day.After 21 days, model evaluation was made through observing the rats weight and sugar consumption experiment.ELISA method was used for determination of the content of the rat serum IL-1 and IL-6. Result After 21 days of stress method,compared with normel group,model group was slow increase of body weight,sugar consumption、the content of serum IL-1 and IL-6 were decreased significantly, and western medicine group and Mongolia medicine Nutmeg Wuwei pills groups could improve significantly the depression of model rats to some extent.Compared with model group, positive control group and Mongolia medicine Nutmeg Wuwei pills groups got significantly derease the content of IL-1 and IL-6 in serum of rats( P <0.05). Conclusion Mongolia medicine Nutmeg Wuwei pills restored the content of IL-1 and IL-6 induced by model group.

关 键 词：肉蔻-5味丸 抑郁模型 大鼠 IL-1 IL-6 

分 类 号：R285.5[医药卫生—中药学]