蝙蝠葛碱对人食管癌细胞Eca-109的增殖和凋亡影响的实验研究  

Experimental study on the inhibitory effect of dauricine on human esophageal cancer cell line Eca-109 and its apoptosis

作　　者：贾红玉 王赫楠 夏风宇 孙艳[1] 刘红利[1] 李姗姗 闫丽丽[1] 徐梅梅[1] 姜东春[1] JIA Hong-yu;WANG He-nan;XIA Feng-yu(Department of Digestive Medicine,The First Hospital of Qinhuangda,Qinhuangdao Hebei 066000,China.)

机构地区：[1]秦皇岛市第一医院消化内科

出　　处：《临床和实验医学杂志》2019年第18期1927-1930,共4页Journal of Clinical and Experimental Medicine

基　　金：河北省卫生和计划生育项目(编号:20171497);秦皇岛市科技支撑计划项目(编号:201602A140)

摘　　要：目的研究蝙蝠葛碱对人食管癌细胞Eca-109的增殖和凋亡的影响。方法 12 h、24 h、48 h采用MMT实验检测0μM、20μM、40μM、80μM的蝙蝠葛碱作用于人食管癌细胞Eca-109增殖的抑制率,并经流式细胞仪对人食管癌细胞Eca-109凋亡周期、早期凋亡率进行检测,同时应用Western blot法对细胞凋亡基因Caspase-3、Bax、Bcl-2表达进行检测。结果与0μM蝙蝠葛碱比较,20~80μM蝙蝠葛碱均能对Eca-109细胞的增殖进行抑制,并细胞生长抑制率随着蝙蝠葛碱浓度增加、作用时间延长而增高,差异有统计学意义(P <0. 05);相较于0μM蝙蝠葛碱,20μM、40μM、80μM蝙蝠葛碱的G1期细胞均增加,并G1期细胞随着蝙蝠葛碱浓度的逐渐增加而不断增多,差异有统计学意义(P <0. 05);G2M期细胞随着蝙蝠葛碱的浓度增加而呈增多趋势;20~80μM蝙蝠葛碱对人食管癌Eca-109细胞凋亡均具有促进作用,且细胞凋亡率随着蝙蝠葛碱应用浓度增加而不断升高,差异有统计学意义(P <0. 05);20~80μM蝙蝠葛碱作用24 h后Caspase-3蛋白活性、Bax蛋白的表达均增强,而Bcl-2蛋白的表达减少,差异有统计学意义(P<0. 05)。结论 20~80μM蝙蝠葛碱能对人食管癌细胞Eca-109增殖进行抑制,促进Eca-109细胞凋亡,并呈剂量、时间依赖性,分析原因可能与上调Caspase-3、Bax蛋白表达、下调Bcl-2表达有关。Objective To study the inhibitory effect of dauricine on human esophageal cancer cell line Eca-109 and its apoptosis, in order to provide information for clinical prevention and treatment of esophageal cancer. Methods The inhibition rate of the growth of human esophageal cancer cell line Eca-109 by different concentrations (0 μM, 20 μM, 40 μM, 80 μM) and different time (12 h, 24 h, 48 h) was studied by MMT test. The apoptosis cycle and early apoptosis rate of human esophageal cancer cell line Eca-109 were detected by flow cytometry. The expressions of apoptosis genes Caspase-3, Bax and Bcl-2 were detected by Western blot. Results 20~80 μM dauricine could inhibit the proliferation of Eca-109 cells, and the cell growth inhibition rate increased with the increase of tarucudine concentration and prolonged action time ( P <0.05). Compared with the control group, the G1 phase cells of different concentrations of dauricine increased, and the G1 phase cells increased with the increasing concentration of dauricine ( P <0.05). The G2M phase cells showed an increasing trend with the increase of the concentration of dauricine;different concentrations of dauricine all promoted the apoptosis of human esophageal cancer Eca-109 cells, and the apoptosis rate increased with the application of dauricine ( P <0.05). The expression of Caspase-3 protein and Bax protein increased after 24 h of 20~80 μM dauricine, while the expression of Bcl-2 protein decreased ( P <0.05). Conclusion Dauricine can inhibit the proliferation of human esophageal cancer cell line Eca-109 and promote the apoptosis of Eca-109 cells in a dose- and time-dependent manner. The reason may be related to up-regulating Caspase-3 and Bax protein expression and down-regulating Bcl-2 expression.

关 键 词：食管癌 蝙蝠葛碱 细胞Eca-109 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]