瑞舒伐他汀通过抑制炎症反应改善心肌梗死大鼠心功能及心肌纤维化  

Rosuvastatin improves cardiac function and myocardial fibrosis by inhibiting inflammation in rats with myocardial infarction

作　　者：朱灿阳 吴丹娜[2] 黎燕峰 ZHU Can-yang;WU Dan-na;LI Yan-feng(Department of Pharmacy , Hainan Maternal and Child Health Hospital,Haikou 570206 y Hainan Province,China;Department of Pharmacy,Hainan Peopler s Hospital, Haikou 570103, Hainan Province, China;Department of Pharmacology, Basic Medical College of Hebei Medical University, Shijiazhuang 050017, HebeiProvince, China)

机构地区：[1]海南省妇女儿童医学中心药学部,海南海口570206 [2]海南省人民医院药学部,海南海口570103 [3]河北医科大学基础医学院药理学教研室,河北石家庄050017

出　　处：《中国临床药理学杂志》2019年第16期1742-1745,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的观察瑞舒伐他汀对心肌梗死(MI)大鼠心功能及心肌纤维化的改善作用。方法根据体重将45只Wistar大鼠分为假手术组、模型组和实验组,每组15只。用结扎大鼠左冠状动脉前降支构建MI大鼠模型,假手术组仅穿线而不结扎。术后第1d实验组开始予以灌胃瑞舒伐他汀2mg·kg-1,模型组和假手术组予以等量0.9%NaCl,每日1次,共4周。用超声心动图检测大鼠心功能,用Masson染色法观察大鼠心肌纤维化及心肌胶原含量的变化,蛋白免疫印迹法检测心肌组织炎性因子表达。结果假手术组、模型组和实验组大鼠心功能指标中左室舒张末期内径(LVEDD)分别为(0.57±0.12),(0.70±0.08),(0.61±0.11)cm;左室收缩末期内径(LVESD)分别为(0.29±0.06),(0.69±0.04),(0.58±0.07)cm;左室短轴缩短分数(LVFS)分别为(48.26±6.95)%,(27.49±6.84)%,(35.16±12.05)%;左室射血分数(LVEF)分别为(72.32±7.32)%,(43.31±7.76)%,(56.43±9.75)%;假手术组、模型组和实验组大鼠梗死区心肌胶原梗死区域(CVF)分别为(3.12±1.26)%,(78.16±1.25)%,(74.12±0.53)%,假手术组、模型组和实验组大鼠心内膜下区CVF分别为(1.02±0.11)%,(2.36±0.42)%,(1.32±0.25)%,间质组织CVF分别为(0.81±0.12)%,(1.69±0.25)%,(1.11±0.03)%;心肌组织中肿瘤坏死因子(TNF)-α蛋白相对表达量分别为0.45±0.11,0.85±0.06,0.56±0.05,白细胞介素(IL)-1β蛋白相对表达量分别为0.35±0.05,0.96±0.12,0.82±0.03,IL-10蛋白相对表达量分别为0.85±0.11,0.36±0.07,0.81±0.12。模型组的上述指标分别与假手术组和实验组比较,差异均有统计学意义(均P<0.05)。结论瑞舒伐他汀可延缓MI大鼠的心肌纤维化改变,改善心功能,与其可调控心肌组织TNF-α,IL-1β,IL-10蛋白表达,抑制炎性反应有关。Objective To observe the effect of rosuvastatin on cardiac function and myocardial fibrosis in rats with myocardial infarction(MI). Methods A total of 24 Wistar rats were assigned to sham-operation group, model group and test group according to their weight, with 8 rats in each group. MI model was established by ligating the anterior descending branch of the left coronary artery, the rats in the sham-operation group was only threaded without ligation. On the Day 1 after operation, the test group was given oral administration of rosuvastatin 2 mg·kg-1 once daily for 4 weeks, while the model group and the sham-operation group were given the equal amount of 0.9%NaCl. Cardiac function was measured by echocardiography,myocardial fibrosis and collagen content were observed by Masson staining,and inflammatory factors proteins expression were detected by Western blotting. Results After drug intervention,the left ventricular end-diastolic diameter( LVEDD) in sham-operation group,model group and test group were( 0. 57 ± 0. 12),( 0. 70 ±0. 08),( 0. 61 ± 0. 11) cm,and left ventricular end-systolic diameter( LVESD) were( 0. 29 ± 0. 06),( 0. 69 ± 0. 04),( 0. 58 ± 0. 07) cm,respectively;left ventricular fractional shortening( LVFS) were( 48. 26 ±6. 95)%,( 27. 49 ±6. 84)%,( 35. 16 ±12. 05)%,respectively;left ventricular ejection fraction( LVEF)were( 72. 32 ± 7. 32)%,( 43. 31 ± 7. 76)%,( 56. 43 ± 9. 75)%,respectively;the myocardial interstitial collagen volume fraction( CVF) in sham-operation group, model group and test group were( 3. 12 ± 1. 26)%,( 78. 16 ±1. 25)%,( 74. 12 ±0. 53)%,respectively;CVF of subendocardial region in sham-operation group,model group and test group were( 1. 02 ± 0. 11)%,( 2. 36 ± 0. 42)%,( 1. 32 ± 0. 25)%,respectively;CVF of interstitial tissue in each groups were( 0. 81 ± 0. 12)%,( 1. 69 ± 0. 25)%,( 1. 11 ± 0. 03)%,respectively;the expression of tumor necrosis factor-α( TNF-α) protein in myocardium were 0. 45 ± 0. 11,0. 85 ± 0. 06,0. 56 ± 0. 05,respectively;the expression of i

关 键 词：急性心肌梗死 瑞舒伐他汀 心肌纤维化 炎性反应 

分 类 号：R972[医药卫生—药品]