HPLC法测定健胃胶囊中芍药苷含量  

Determination of Paeoniflorin in JianWei Capsules by HPLC

作　　者：赵南 肖锦新 李星 陈运琴 孙田甜 陈红羽 ZHAO Nan;XIAO Jin-xin;LI Xing;CHEN Yun-qin;SUN Tian-tian;CHEN Hong-yu(Guizhou Bailing Group Pharmaceutical Co.,Ltd.,Aushun 561000,China)

机构地区：[1]贵州百灵企业集团制药股份有限公司

出　　处：《海峡药学》2019年第8期115-117,共3页Strait Pharmaceutical Journal

基　　金：贵州省科技厅中药现代化黔科合中药字[2011]5074号

摘　　要：目的建立HPLC法测定健胃胶囊中活性成分芍药苷的测定方法。方法以十八烷基硅烷键合硅胶为填充剂,以乙腈-0.1%磷酸溶液(14∶86)为流动相,检测波长为230nm。结果在132.19~1850.69ng范围内,芍药苷的峰面积与进样量呈现良好的线性关系(r=0.9999)。平均回收率为100.15%,RSD%为0.91%。结论本方法操作简单,重现性良好,该方法可用于健胃胶囊中芍药苷的含量测定。OBJECTIVE To establish a HPLC method for the determination of Paeoniflorin in JianWei Capsules. METHODS An Elite Sinochrom ODS-BP (5μm,4.6mm×250mm) column was adopted.The mobile phase was consisted of acetonitrile--0.1% phosphoric acid solution (14∶ 86),and the Detection wavelength was 262nm. RESULTS The calibration curve of peak area integral value of calibration was linear in the range of 132.19~1850.69ng (R=0.9999),the average recovery ratio was 100.15%(RSD=0.91%). CONCLUSION The method can be used for the determination of paeoniflorin in JianWei capsule due to its simplicity,repeatability and reliability.

关 键 词：HPLC法 芍药苷 含量测定 健胃胶囊 

分 类 号：R927[医药卫生—药学]