基于高通量测序技术的玉米不同生育时期土壤细菌多样性变化  

Variations of Soil Bacterial Diversity at Different Growth Stages in Maize Based on High-throughput Sequencing

作　　者：李丹[1] 靳鲲鹏[1] 李小霞 韩文清[1] 曹晋军[1] 刘永忠[1] 李万星[1] LI Dan;JIN Kunpeng;LI Xiaoxia;HAN Wenqing;CAO Jinjun;LIU Yongzhong;LI Wanxing(Institute of Millet,Shanxi Academy of Agricultural Sciences,Changzhi 046011,China)

机构地区：[1]山西省农业科学院谷子研究所

出　　处：《山西农业科学》2019年第9期1569-1572,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省重点研发计划重点项目(201703D211002-6);山西省农业科学院有机旱作农业研发专项(YCX2017D2404)

摘　　要：为探明玉米关键生育时期土壤细菌多样性的动态变化,基于Illumina HiSeq 2500高通量测序技术测定细菌16SrRNAV3+V4区序列,分析玉米4个关键生育时期土壤细菌的菌群结构及多样性。结果表明,在玉米4个关键生育时期,分别检测到42,37,39,37个细菌门,其中,变形菌门是研究区土壤中的优势细菌类群,占有绝对优势,其次依次是酸杆菌门、放线菌门、绿弯菌门、拟杆菌门、芽单胞杆菌门;土壤细菌多样性随着生育时期的进程而变化,苗期土壤细菌多样性最高,成熟期最低。To study the dynamic change of soil bacteria diversity during the key growth period of maize, the Illumina HiSeq 2500 high-throughput sequencing technology was used to determine the 16S rDNA V3+V4 region sequence of bacteria, and to analyse the structure and diversity of soil bacteria in four key growth stages of maize. The results showed that 42, 37, 39, 37 were detected in 4 samples at phylum stage during the whole growth period of maize, Proteobacteria was the dominant bacteria at phylum level, followed by Acidobacteria, Actinobacteria, Chloroflexi, Bcateroidetes, Gemmatimonadetes. The diversity of soil bacteria varied during the different growth period, soil bacteria diversity in seedling period was the highest and the maturity was the lowest.

关 键 词：玉米 不同生育时期 细菌 多样性 

分 类 号：S154.381[农业科学—土壤学]