RP-HPLC法同时测定车前子中4种主要成分的含量  

Simultaneous determination of four main components in Plantaginis Semen by RP-HPLC

作　　者：吴丹[1] WU Dan(Key Laboratory of Chinese Materia Medica(Ministry of Education),Heilongjiang University of Chinese Medicine,Heilongjiang Key Laboratory of TCM Pharmacodynamic Material Bases,Heilongjiang 150040)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室,黑龙江省中药天然药物药效物质基础研究重点实验室

出　　处：《中国食品添加剂》2019年第8期158-162,共5页China Food Additives

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB531801)

摘　　要：目的:建立反相高效液相色谱法同时检测车前子中京尼平苷酸、车前素、麦角甾苷和异麦角甾苷4种主要成分的含量。方法:采用Dikma Diamonsil C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇(A)-0.05‰甲酸水溶液(B),梯度洗脱(0~30min,5%~90%A),流速为1.0mL·min^-1,检测波长为205nm,柱温25℃。结果:京尼平苷酸、车前素、麦角甾苷和异麦角甾苷之间的分离度良好;各成分质量与峰面积在测定范围内均呈良好的线性关系(r>0.9998);平均加样回收率(n=6)分别为98.17%(RSD=3.55%)、104.36%(RSD=3.77%)、98.97%(RSD=4.35%)和97.63%(RSD=3.12%)。结论:该实验开发的方法可成功应用于车前子中4种主要成分的分析。Objective:To establish a high-performance liquid chromatography method for simultaneous determination of four main components including geniposidic acid,plantagoguanidinic acid,verbascoside and isoverbascoside in Plantaginis Semen.Methods:The Dikma Diamonsil C18(250mm×4.6mm,5μm)column was used with the mobile phase of methanol(A)-0.05‰Formic acid(B)for gradient elution(0~30min,5%~90%A),at a flow rate of 1.0 mL·min^-1.The detection wavelength was 205 nm,and the column temperature was at 25℃.Results:The geniposidic acid,plantagoguanidinic acid,verbascoside and isoverbascoside were well separated;the quality and peak area of each component in the range of detection revealed a good linear relationship(r>0.9998).The average recoveries(n=6)were 98.17%(RSD=3.55%),104.36%(RSD=3.77%),98.97%(RSD=4.35%)and 97.63%(RSD=3.12%).Conclusion:The high-performance liquid chromatography method developed was successfully used to analyze four main effective components in Plantaginis Semen.

关 键 词：车前子 反相高效液相色谱法 京尼平苷酸 车前素 麦角甾苷 异麦角甾苷 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS202.1[轻工技术与工程—食品科学与工程]