ICU多重耐药铜绿假单胞菌oprD2基因缺失的研究  

Analysis of the absence of oprD2 gene of multidrug-resistant pseudomonas aeruginosa in ICU

作　　者：彭咏麟 刘立君 PENG Yonglin;LIU Lijun(Department of Clinical Laboratory,Hengyang Central Hospital,Hengyang,Hunan 421001,China)

机构地区：[1]湖南省衡阳市中心医院检验科

出　　处：《检验医学与临床》2019年第17期2453-2454,2458,共3页Laboratory Medicine and Clinic

基　　金：湖南省卫生计生委基金项目(湘卫计委B2015-158)

摘　　要：目的探讨ICU多重耐药铜绿假单胞菌(MDRP)外膜孔蛋白oprD2基因的缺失情况。方法通过ICU60株MDRP采用K-B法筛选出对亚胺培南耐药的菌株;采用PCR法检测对亚胺培南耐药的MDRPoprD2基因。结果60株MDRP对亚胺培南耐药有37株,耐药率61.7%(37/60);37株对亚胺培南耐药的MDRP有30株缺失oprD2基因,缺失率81.1%(30/37)。结论ICUMDRP对碳青霉烯类药物耐药严重;细菌外膜孔蛋白oprD2基因缺失导致菌体包膜通透性改变是ICUMDRP耐药机制的主要原因。Objective To investigate the absence of the outer membrane porin oprD2 gene of sixty strains of multi-drug resistant pseudomonas aeruginosa(P.aeruginosa)in the ICU of this region.Methods A total of 60 strains of multidrug-resistant P.aeruginosa in the ICU were screened for imipenem-resistant strains by K-B method.The absence of oprD2 gene of the selected multidrug-resistant P.aeruginosa were detected by PCR.Results Thirty-seven of 60 strains of multidrug-resistant P.aeruginosa were resistant to imipenem,and the resistance rate was 61.7%(37/60).30 of the 37 imipenem-resistant strains of oprD2 gene were absent,with an absence rate of 81.1%(30/37).Conclusion The multi-drug resistant P.aeruginosa in the ICU is highly resistant to carbapenems.The absence of bacterial outer membrane pore protein oprD2 gene leads to the change of bacterial envelope permeability in the ICU might be the main aspects of bacterial resistance mechanisms.

关 键 词：铜绿假单胞菌 多重耐药 重症监护室 外膜蛋白 

分 类 号：R378.991[医药卫生—病原生物学]