基于Rho/ROCK信号通路的针刺对高脂血症模型大鼠血管内皮保护机制研究  

Effects of Acupuncture on Endothelial Function through The Rho/ROCK Signaling Pathway in Rats with Hyperglycaemia

作　　者：陶欢 刘盛菲 凌敏[1] TAO Huan;LIU Sheng-fei;LING Min(Guangzhou University of Chinese Medicine, Guangzhou 510006, China)

机构地区：[1]广州中医药大学

出　　处：《中国中医基础医学杂志》2019年第8期1134-1136,1142,共4页JOURNAL OF BASIC CHINESE MEDICINE

摘　　要：目的:通过针刺对高脂血症模型大鼠进行干预,观察相关指标变化探讨其改善血管内皮功能的机制。方法:将SD大鼠按随机数字表法分为空白组、模型组、针刺组各10只,模型组、针刺组大鼠采用高脂乳剂灌胃方法建立高脂血症大鼠模型,空白组、模型组只固定不做其他治疗,针刺组采用"脂三针"治疗3周。实验过程中观察各组大鼠一般状态及体质量变化,实验结束测定血清、血脂水平,光镜下观察主动脉形态学改变,采用Western blot法检测主动脉RhoA、ROCK2、MLCP蛋白表达水平。结果:与模型组比较,针刺组大鼠一般状态明显改善,体质量降低,血脂TC、TG、LDL-C含量显著降低,HDL-C明显含量升高,血清NO含量升高、ET-1含量降低,主动脉病理损伤程度有较好改善, RhoA、ROCK2、MLCP蛋白表达显著降低,差异有统计学意义。结论:针刺可降低血脂,调节血管舒缩因子含量,降低相关蛋白表达,改善血管内皮损伤,推测其机制或许与抑制Rho/ROCK通路激活有关。Objective: The intervention of acupuncture on hyperlipidemic rats was carried out to observe the changes of related indicators and explore the mechanism of improving vascular endothelial function. Methods: SD rats were randomly divided into blank group, model group and acupuncture group, 10 rats in each group. The rats in the model group and the acupuncture group were treated with high fat emulsion to establish the hyperlipidemia rat model. The blank group and the model group were only fixed without any other treatment. The acupuncture group was treated with "fat three needles" for 3 weeks. The general state performance and body weight change were observed during the experiment. After the experiment, serum and blood lipid indexes were measured and the morphological changes of aorta were observed by the light microscope. The expression of RhoA, ROCK2 and MLCP protein in aorta was detected by Western blot. Results Compared with the model group, the general state of the rats in the acupuncture group was obviously improved, the weight decreased, the content of TC, TG and LDL-C in the blood lipid decreased significantly, the content of HDL-C increased, the content of NO in the serum increased, the content of ET-1 decreased, the degree of pathological injury of the aorta was better, the expression of RhoA, ROCK2 and MLCP decreased significantly. Conclusion: Acupuncture may reduce blood lipid, regulate the content of vasoconstriction factor, reduce the expression of related proteins and improve vascular endothelial injury, which may be related to the inhibition of the activation of Rho/ROCK signaling pathway.

关 键 词：针刺 高脂血症模型大鼠 血管内皮 Rho/ROCK通路 

分 类 号：R246.1[医药卫生—针灸推拿学]