川芎嗪对脊髓缺血再灌注损伤模型大鼠细胞自噬相关蛋白的影响  

Effects of ligustrazine on autophagy-related proteins after spinal cord ischemia-reperfusion injury

作　　者：郭晓辉 郑怀亮 李艳侠 夏祖辉 倘艳锋 李磊 Guo Xiaohui;Zheng Huailiang;Li Yanxia;Xia Zuhui;Tang Yangfeng;Li Lei Luoyang(Orthopedic-Traumatological Hospital of Henan (Henan Orthopaedic Hospital ), Luoyang 471000, China)

机构地区：[1]河南省洛阳正骨医院(河南省骨科医院),471000

出　　处：《国际中医中药杂志》2019年第8期843-846,共4页International Journal of Traditional Chinese Medicine

基　　金：河南省中医药科学研究专项课题(2015ZY02067).

摘　　要：目的探讨川芎嗪对脊髓缺血再灌注损伤模型大鼠细胞自噬相关蛋白Beclin l、微管相关蛋白1轻链3(microtubule-assaiated protein 1 light chain 3, LC3)、死骨片1(sequestosome 1, P62)的影响。方法将48只SD大鼠按随机数字表法分为假手术组、模型组、川芎嗪组和抑制剂组,每组12只。川芎嗪组大鼠腹腔注射川芎嗪注射液0.16 mg/kg,抑制剂组腹腔注射3-甲基腺嘌呤注射液0.015 mg/kg,假手术组和模型组腹腔注射等体积生理盐水。给药后,除假手术组外,其余各组大鼠制备脊髓缺血再灌注模型。再灌注后3、6 h进行行为学评分,采用免疫组化染色法检测脊髓组织Beclin l、LC3、P62表达变化。结果造模后3、6 h,与模型组比较,川芎嗪组大鼠行为学评分[3 h:(2.33±0.58)分比(0.67±0.58)分;6 h:(3.33±0.58)分比(1.33±0.58)分]升高(P<0.05);脊髓组织Beclinl积分光密度[3 h:(348.00×104± 0.27×104)比(659.00×104±0.11×104);6 h:(38.00×104±0.19×104)比(557.00×104±0.26×104)]、LC3积分光密度[3 h:(357.00×104±0.48×104)比(686.00×104±0.33×104);6 h:(334.00×104±0.51×104)比(673.00×104±0.22×104)]、P62[3 h:(357.00×104±0.48×104)比(830.00×104±0.48×104);6 h:(315.00×104±0.12×104)比(591.00×104±0.36×104)]表达降低(P<0.05)。结论川芎嗪对脊髓缺血再灌注模型大鼠细胞自噬具有调节作用,可发挥神经细胞保护作用。Objective To investigate the effect of ligustrazine on autophagy-related proteins Beclin l, LC3 and P62 after spinal cord ischemia-reperfusion injury. Methods A total of 48 SD rats were randomly divided into sham operation group, model group, ligustrazine group and 3-MA group. The rats were intraperitoneally injected with ligustrazine injection 0.16 mg/kg in the Ligustrazine group, the rats were intraperitoneally injected with 3-methyladenine injection 0.015 mg/kg in the inhibitor group, and the rats were intraperitoneally injected with normal saline of equal volume in the sham operation group and model group. Spinal cord ischemia-reperfusion model was established in all groups except sham-operated group after administration. After molding behavioral scores were scored after 3 and 6 hours of ischemia, and the expression of Beclin l, LC3 and P62 was detected by immunohis-tochemistry. Results After 3 and 6 hours, compared with the model group, the behavioral score (3 h: 2.33 ± 0.58 vs. 0.67 ± 0.58, 6 h: 3.33 ± 0.58 vs. 1.33 ± 0.58) of the rats in ligustrazine group significantly increased (P<0.05). Compared with the model group, the expression of Beclinl (3 h: 348.00×104 ± 0.27×104 vs. 659.00×104 ± 0.11×104;6 h: 38.00×104 ± 0.19×104 vs. 557.00×104 ± 0.26×104), LC3 (3 h: 357.00×104 ± 0.48×104 vs. 686.00×104 ± 0.33×104;6 h: 334.00×104 ± 0.51×104 vs. 673.00×104 ± 0.22×104), P62 (3 h: 357.00×104 ± 0.48×104 vs. 830.00×104 ± 0.48×104;6 h: 315.00×104 ± 0.12×104 vs. 591.00×104 ± 0.36×104) in ligustrazine group were significantly decreased (P<0.05). Conclusions The ligustrazine may regulate autophagy in two directions and protect nerve cells.

关 键 词：川芎嗪 脊髓缺血 再灌注损伤 BECLIN-1 大鼠 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]