水稻与弱毒丝核菌互作中免疫相关基因表达研究  

Expression of rice immunity genes involved in interaction between attenuated Rhizoctonia solani and rice

作　　者：刘列 张世梅 王明环 张杰 杨敏[1] 杨静[1] 李成云[1] 杨根华[1] 董文汉 Liu Lie;Zhang Shimei;Wang Minghuan;Zhang Jie;Yang Min;Yang Jing;Li Chengyun;Yang Genhua;Dong Wenhan(State Key Laboratory for Conversation and Utilization of Bio -resources in Yunnan/ Yunnan Agricultural University, Kunming Yunnan 650201, China;Science and Technology Bureau of Yunnan Agricultural University, Kunming Yunnan 650201, China)

机构地区：[1]云南农业大学/省部共建云南生物资源保护与利用国家重点实验室,云南昆明650201 [2]云南农业大学科技处,云南昆明650201

出　　处：《中国植保导刊》2019年第8期5-14,共10页China Plant Protection

基　　金：国家重点研发计划(2017YFD0200400);国家自然科学基金(31160352,31360423)

摘　　要：水稻纹枯病的病原菌为立枯丝核菌(Rhizoctonia solani Kǜhn)。用10株弱毒丝核菌提前接种水稻后,再接种纹枯病病原菌,观察其发病症状,并用实时荧光定量RT-PCR方法研究了接种不同处理后的水稻9个抗性基因表达情况,筛选出参与水稻纹枯病抗病过程的基因和能使水稻提前获得系统抗性的弱毒丝核菌菌株。结果显示,参与水稻诱导抗性的抗性基因主要为PR10a、ORK10、NAC4、WRKY53、WRKY71。大部分弱毒双核丝核菌能诱导水稻抗性基因表达,诱导效果最好的弱毒丝核菌菌株是②BS-J-06-8-1、④chd-YT-3-5和⑨DL-YT-06-4-9。The pathogen of rice sheath blight is Rhizoctonia solani Kǜhn. In this study, 10 strains of attenuated R. solani were inoculated with rice in advance. And the pathogen was inoculated and the symptoms were observed. The expression of 9 resistance genes in rice treated with different treatments was studied with RT-PCR, and screening was carried out for the genes involved in resistance process of rice sheath blight and the strains of R. solani which can obtain the system resistance in advance. The results showed that the main-involved genes were PR10 a, ORK10, NAC4, WRKY53 and WRKY71. Most of the attenuated R. solani can induce the expression of rice resistance genes, and the strains with the best induction effect were:②BS-J-06-8-1,④chd-YT-3-5 and ⑨DL-YT-06-4-9.

关 键 词：弱毒双核丝核菌 水稻纹枯病 诱导抗性 抗性相关基因 RT-PCR 

分 类 号：S435[农业科学—农业昆虫与害虫防治] S435.111.42[农业科学—植物保护]