麦洼牦牛PRL基因多态与产奶性状的关联分析  

Correlation Analysis on Polymprphism of Prolactin Gene with Milk Performance Traits in Maiwa Yak

作　　者：李铸 何世明 吴锦波 艾鷖 蹇尚林 刘建 雍军 LI Zhu;HE Shi-ming;WU Jin-bo(Institute of Animal Science and Technology of Aba Tibetan and Qiang Autonomous Prefecture, Hongyuan,Sichuan 624402)

机构地区：[1]阿坝藏族羌族自治州畜牧科学技术研究所,四川红原624402 [2]西南民族大学青藏高原研究院,四川成都610041 [3]阿坝藏族羌族自治州畜牧工作站,四川马尔康624099

出　　处：《安徽农业科学》2019年第18期108-110,共3页Journal of Anhui Agricultural Sciences

基　　金：四川省科技厅科技支撑计划项目(2015FZ0024)

摘　　要：研究旨在了解麦洼牦牛催乳素(PRL)基因的单核苷酸多态性(SNP),探究与牦牛产奶性能相关的分子标记。通过对PCR产物进行直接测序筛选麦洼牦牛 PRL 基因的SNP位点,运用DNAMAN和SPSS等软件对 PRL 基因型与产奶性状进行了统计分析。结果表明,位于 PRL 基因996 bp处发现一个疑似SNP位点,该位点T碱基缺失突变;对比麦洼牦牛不同 PRL 基因型的产奶性状,突变型个体的 153 d 产奶量、乳蛋白质率、乳糖率和乳中的体细胞数均下降( P >0.05),而乳脂率则显著升高(P <0.05),暗示该处碱基T碱基缺失对乳中脂肪含量有显著影响。In order to study the single nucleotide polymorphism (SNP) of the Maiwa yak prolactin gene and to explore the molecular markers related to milk performance traits of yak. We used the direct PCR sequencing method to screen the SNPs, and analyzed the correlation between prolactin genotype and milk performance traits by employing statistical softwares such as DNAMAN and SPSS.The results showed that we found one SNP site which located at 996 bp of prolactin gene and it’s T-base deletion mutation at this site. By comparing the milk performance traits of different prolactin gene types, the 153 d milk yield, lactoprotein, milk sugar and somatic cell number in milk of mutant individuals were lower than normal genotype individuals ( P> 0.05), while the milk fat increased significantly ( P <0.05). It was suggested that the absence of T-base had a significant effect on the fat content of Maiwa yak milk.

关 键 词：牦牛 PRL 基因 SNPS 产奶性状 关联分析 

分 类 号：S823.85[农业科学—畜牧学]