12种中草药乙醇提取液对香蕉根结线虫的毒杀效果  

Toxic Effect of Ethanol Extracts from 12 Kinds of Chinese Herbal Medicines on Root-knot Nematodes of Banana

作　　者：李春 赵东兴 张建春 李芹 LI Chun;ZHAO Dong-xing;ZHANG Jian-chun(Honghe Institute of Tropical Agricultural Sciences of Yunnan, Hekou,Yunnan 661399)

机构地区：[1]云南省红河热带农业科学研究所

出　　处：《安徽农业科学》2019年第18期150-151,166,共3页Journal of Anhui Agricultural Sciences

基　　金：云南省科技计划项目“植物提取液对河口地区香蕉根结线虫的毒杀作用研究”(2017FD035)

摘　　要：[目的]研究12种中草药乙醇提取物对香蕉根结线虫的毒杀活性,为香蕉根结线虫的防治提供参考。[方法]12种中草药用乙醇提取浓缩后,将各提取物配成5%的溶液,加入经人工培养的二龄线虫60条,分别在24、48、72h后于显微镜下观察线虫死亡数量,并计算校正死亡率。[结果]艾叶、鸡屎藤、苦豆子、牛心朴子草、细辛和紫茎泽兰的乙醇提取物对香蕉根结线虫有较强的毒杀活性,穿心莲、川芎、银杏叶、叶下珠提取物的杀线虫活性中等,马齿笕、黄芪提取物的杀线虫活性较弱,且随着时间的延长,各处理的毒杀活性增强。[结论]艾叶、鸡屎藤、苦豆子、牛心朴子草、细辛和紫茎泽兰对香蕉根结线虫有较强的毒杀活性。[Objective] The toxicity of ethanol extracts from 12 Chinese herbal medicines on banana root-knot nematodes was studied to provide reference for the control of banana root knot nematodes.[Method]The extracts were prepared into 5% solution after by ethanol extract and concentration of 12 kinds of Chinese herbal medicines. And then 60 2nd instar larvae of artificial culture were added. The number of dead nematodes was observed under the microscope after 24, 48 and 72 h, and the adjusted mortality was calculated.[Result]Ethanol extracts from Artemisia argyi Levl.et Vant., Paederia scandens (Lour.) Merr., Sophora alopecuroides L., Cynanchun Komarovii AL., Asarum sieboldii Miq., Eupatorium odoratum L. had strong toxic activity on banana root-knot nematodes.;the activities of extracts from Andrographis paniculata (Burm. f.) Nees, Ligusticum chuanxiong Hort., Ginkgobiloba, Phyllanthus urinaria L were medium;and extracts from Portulaca oleracea L. and Astragalus membranaceus (Fisch.) Bunge. were weak. And the activities were increased with treatment time.[Conclusion]Ethanol extracts from Artemisia argyi Levl.et Vant., Paederia scandens (Lour.) Merr., Sophora alopecuroides L., Cynanchun Komarovii AL., Asarum sieboldii Miq., Eupatorium odoratum L. has strong toxic activity on banana root-knot nematodes.

关 键 词：中草药 乙醇 香蕉 根结线虫 毒杀活性 

分 类 号：S432.4[农业科学—植物病理学]