HPLC法测定补骨胶囊中柚皮苷的含量  

Determination of Naringin in Bugu Capsule by HPLC

作　　者：王亮 谢宇 罗君 薛鑫宇 唐靖雯 张丽艳 Wang Liang;Xie Yu;Luo Jun;Xue Xinyu;Tang Jingwen;Zhang Liyan(Guiyang Collage of Traditional Chinese Medicine,Guiyang 550025 Guiyang,China;Guizhou Guangjitang Pharmaceutical Company Limited,Guiyang 550014,China;First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine (TCM),Guiyang 550001 ,China;Cuizhou Weimen Pharmaceutical Co.,Ltd.,Cuiyang 550004,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]贵州广济堂药业有限公司,贵州贵阳550014 [3]贵州中医药大学第一附属医院,贵州贵阳550001 [4]贵州威门药业股份有限公司,贵州贵阳550004

出　　处：《亚太传统医药》2019年第8期77-80,共4页Asia-Pacific Traditional Medicine

基　　金：贵州省国内一流建设学科(中药学)项目(GNYL[2017]008号);贵州省科技支撑计划项目(黔科合支撑[2018]2772);贵州省科技厅贵阳中医学院第一附属医院联合基金(黔科合LH字[2015]7801);国家中医药管理局中药特色技术传承人才项目(国中医药办人教函[2018]204号);贵阳国家高新技术产业开发区管理委员会科技型中小企业技术创新基金(GXCX2015-012)

摘　　要：目的:建立补骨胶囊中柚皮苷的含量测定方法。方法:采用HPLC法建立补骨胶囊中柚皮苷含量测定方法。采用Ultimate XB-C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-醋酸-水(35∶4∶65)为流动相,流速1.0 mL·min^-1,检测波长283 nm,柱温30℃,进样量10μL。结果:柚皮苷在2.345~75.019μg/mL范围内线性关系良好,回归方程为Y=17.85X+5.500 9,r=0.999 9;精密度、重复性、稳定性试验RSD均小于2.0%;加样回收率在90%~108%之间(RSD=1.87%,n=6)。结论:该方法简便准确、重复性好、灵敏度高、含量测定方法稳定可行,适用于补骨胶囊中柚皮苷含量测定,可更好地控制其药品质量。Objective:To establish determination of naringin content.Methods:Establish a method for determination of naringin content in Bugu capsules by HPLC.The HPLC separation was performed on Ultimate XB-C18(4.6 mm×250 mm,5 μm)column with a mixture methanol-acetic acid-water(35:4:65)as the mobile phase, at a flow rate of 1 mL/min.Detection wavelength was 283 nm and column temperature was 30 ℃.The injection volume was 10 μL.Results:Naringin has a good linear relationship in the range of 2.345~75.01 μg/mL,the regression equation was Y=17.85X+5.500 9(r=0.999 9);RSDs of precision, stability and reproducibility tests were<2%;Sample recovery limit is between 90% and 108%.(RSD is 1.87%,n=6).Conclusion:The method is simple, accurate, reproducible, sensitive, stable and feasible.It is suitable for the determination of naringin in Bugu capsule and can be used to control the quality of naringin.

关 键 词：补骨胶囊 柚皮苷 HPLC法 含量测定 

分 类 号：R284.2[医药卫生—中药学]