非洲猪瘟病毒基因缺失疫苗株的构建和免疫保护特性  

Construction and immunoprotective characterization of gene deleted African swine fever virus vaccine candidates

作　　者：张艳艳 陈腾 张静远 齐宇 缪发明 薄宗义 王立冬 郭晓宇[3] 周鑫韬 杨金梅 王晓虎 高玉龙 汪春雨 鲍晨沂 米立娟 孙雪霏 冯娜 杨金金 王承宇 万忠海 李记平 孟轲音 田多 李楠 王述超 王颖 张菲 张锦霞 蒋依倩 刘晔 张守峰 张中洋 钱莺娟[6] 朱鸿飞[3] 高玉伟 陈鸿军[2] 李金祥[7] 扈荣良 ZHANG Yan-yan;CHEN Teng;ZHANG Jing-yuan;QI Yu;MIAO Fa-ming;BO Zong-yi;WANG Li dong;GUO Xiao-yu;ZHOU Xin-tao;YANG Jin-mei;WANG Xiao-hu;GAO Yu long;WANG Chun-yu;BAO Chen-yi;MI Li-juan;SUN Xue-fei;FENG Na;YANG Jin-jin;WANG Cheng-yu;WAN Zhong-hai;LI Ji-ping;MENG Ke-yin;TIAN Duo;LI Nan;WANG Shu-chao;WANG Ying;ZHANG Fei;ZHANG Jin-xia;JIANG Yi-qian;LIU Ye;ZHANG Shou-feng;ZHANG Zhong-yang;QIAN Ying-juan;ZHU Hong-fei;GAO Yu-wei;CHEN Hong-jun;LI Jin-xiang;HU Rong-liang(College of veterinary medicine,Nanjing Agricultural University,Nanjing 210095,China 7.Chinese Academy of Agricultural Sciences,Beijing 100081,China;Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China;Beijing Veteri nary Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)

机构地区：[1]军事科学院军事医学研究院军事兽医研究所,吉林长春130122 [2]中国农业科学院上海兽医研究所,上海200241 [3]中国农业科学院北京畜牧兽医研究所,北京100193 [4]吉林和元生物工程股份有限公司,吉林松原138000 [5]广东省农业科学院动物卫生研究所,广东广州510640 [6]南京农业大学动物医学学院,江苏南京210095 [7]中国农业科学院,北京100081

出　　处：《中国兽医学报》2019年第8期1421-1427,共7页Chinese Journal of Veterinary Science

基　　金：国家重点研发计划资助项目(2018YFC0840404);国家重点研发计划资助项目(2017YFD0502300);国家重点研发计划资助项目(2017YFD0502302)

摘　　要：以我国首例非洲猪瘟疫情中分离的流行毒株(SY18)为亲本株构建了多基因家族(MGF)基因和CD2v基因缺失的非洲猪瘟病毒基因缺失疫苗候选株,针对基因缺失株的安全性和免疫保护效果进行了特性研究。结果显示,MGF和CD2v双基因缺失株(ASFV SY18ΔMGF/ΔCD2v)对猪安全,接种猪能够100%抵抗亲本强毒株SY18株的攻击,对照猪全部死亡;说明该毒株可作为非洲猪瘟疫苗候选株。The multi-gene family(MGF) gene-and CD2 v gene-deleted vaccine candidates derived from the SY18 strain of the first African swine fever epidemic in China were constructed and characterized.It was demonstrated that the candidate with double deletions of MGF and CD2v gene was proved to be safe and 100%immunoprotective against the challenge of its parental virulent isolate SY18 for the vaccinated pigs,while all the controls died.This indicates that the double genes deleted virus ASFV SY18ΔMGF/ΔCD2 vis a potential vaccine candidate for African swine fever.

关 键 词：非洲猪瘟病毒 基因缺失 MGF基因 CD2v基因 疫苗候选株 安全性 免疫保护 

分 类 号：S852.65[农业科学—基础兽医学]