猪伪狂犬病病毒变异株(Fujian-LY)人工感染小鼠动物模型的建立  

Establishment of mouse model artificially infected with porcine pseudorabies virus variant (Fujian-LY)

作　　者：范克伟[1,2] 何沙 林青青 郑琳[1,3] 杨守深 刘建奎 林宏彬[1] 宋洲洋 余佳[1,3] 戴爱玲 杨小燕 FAN Ke-wei;HE Sha;LIN Qing-qing;ZHENG Lin;YANG Shou-shen;LIU Jian-kui;LIN Hong-bin;SONG Zhou-yang;YU Jia;DAI Ai-ling;YANG Xiao-yan(College of Life Sciences,Longyan University,Longyan,Fujian 364012,China;Fujian Engineering Research Center for Swine Disease Control and Prevention,Longyan,Fujian 364012,China;College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]龙岩学院生命科学学院,福建龙岩364012 [2]福建省家畜传染病防治与生物技术重点实验室,福建龙岩364012 [3]福建农林大学动物科学学院,福建福州350002

出　　处：《中国兽医学报》2019年第8期1435-1440,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金青年科学基金资助项目(31602048);福建省科技厅自然科学基金资助项目(2016J01712);上杭县奇迈科技创新基金资助项目(2017SHQM06);国家级大学生创新创业训练计划基金资助项目(201711312001);福建省大学生创新创业训练计划基金资助项目(201811312054);龙岩学院校级科技创新团队建设基金资助项目(岩学院科研[2018]13号)

摘　　要：为建立猪伪狂犬病病毒(PRV)变异株人工感染小鼠动物模型,将本实验室分离鉴定的猪PRV变异株(Fujian-LY)进行连续10倍稀释后,对6周龄SPF级BALB/c小鼠进行腹股沟皮下接种攻毒,每个稀释度接种5只小鼠,测定其LD50,观测小鼠感染、致病的多项指标,试验期为7 d。结果显示,该毒株对小鼠的LD50为3.7×103 TCID50/0.1 mL,在不同的感染剂量下各攻毒组小鼠的临床症状、死亡率等各项指标差异明显,其中以2.3×105 TCID50的攻毒剂量接种后小鼠未发生急性死亡,且能表现出以神经症状为主的典型伪狂犬病症状;病理剖检可见发病小鼠脑膜充血,肺脏出血,胸腺、脾脏严重萎缩等病理变化;病理组织学检查结果显示该毒株对攻毒小鼠全身多个重要组织器官均造成了严重的病理损伤,同时采用PCR及免疫组化的方法在这些组织内均能检测到PRV抗原。结果表明,本研究已成功建立了PRV变异株感染小鼠动物模型。In order to establish an artificially infected mouse model of the pseudorabies virus variant strain,the pseudorabies virus variant(Fujian-LY) was serially diluted to 10-fold,and the BALB/c mice(6 weeks-old,SPF level) were challenged with the virus by inguinal inoculation,5 mice were inoculated with each dilution to measure LD50,and many indicators of infection and pathogenesis of the mice were observed within 7 days.The test results showed that the LD50 of the strain was 3.7×103 TCID50/0.1 mL in mice.The clinical symptoms and mortality of mice with different infection doses each group were significantly different.The mice did not display acute death and could show typical pseudorabies symptoms such as neurological symptoms after infection with 2.3×105 TCID50 challenge doses.The necropsy results of diseased mice showed that brain hyperemia,lung hemorrhage,and severe atrophy of thymus and spleen.The pathological examination showed that the strain caused severe pathological damage to many important tissues and organs of the mice.At the same time,PRV antigen could be detected in these tissues by PCR and immunohistochemistry.In conclusion,the mouse model infected with porcine pseudorabies virus variant had been successfully established.

关 键 词：猪 伪狂犬病病毒 变异株(Fujian-LY) 小鼠模型 

分 类 号：S852.65[农业科学—基础兽医学]