辽宁省规模化猪场生产链中沙门菌优势血清型及其主要毒力因子分布  

Distribution of dominant serotypes and major virulence factors of Salmonella in the production chain of large-scale pig farms in Liaoning

作　　者：崔苗苗 刘全 董筱萍 刘静[3] 刘英姿 高慎阳 周宇 张利勃 周铁忠 CUI Miao-miao;LIU Quan;DONG Xiao-ping;LIU Jing;LIU Ying-zi;GAO Shen-yang;ZHOU Yu;ZHANG Li-bo;ZHOU Tie-zhong(College of Animal Husbandry and Veterinary Medicine,Jinzhou Medical University,Jinzhou,Liaoning 121001,China;Liaoning AgriculturalDevelopment Service Center,Shenyang 110032,China;Yixian Animal Disease Prevention and Control Center,Jinzhou,Liaoning 121000,China;Liaoning Yangjciang Agriculture and AnimalHusbandry Co.,Ltd. 9 Shenyang 110300,China)

机构地区：[1]锦州医科大学畜牧兽医学院,辽宁锦州121001 [2]辽宁省农业发展服务中心,辽宁沈阳110032 [3]义县动物疫病预防控制中心,辽宁锦州121000 [4]辽宁扬翔农牧有限公司,辽宁沈阳110300

出　　处：《中国兽医学报》2019年第8期1540-1544,1565,共6页Chinese Journal of Veterinary Science

基　　金：辽宁省自然科学基金资助项目(2015020784)

摘　　要：为了解辽宁省猪沙门菌流行菌株主要血清型毒力因子的分布特征及致病性,依据猪沙门菌属保守基因、多重PCR引物和血清凝集试验,从辽宁省选择有代表性的规模化养猪场、下游生猪屠宰场和肉类市场采集肠道、饲料和污水、猪肉等样品进行猪沙门菌及其血清型鉴定。应用猪沙门菌14种主要毒力因子特异性基因扩增检测方法,检测所分离到的不同血清类型猪链球菌的毒力因子分布情况,并应用小鼠攻毒试验和病理学技术对其致病性进行观察。结果显示:采集的412个样品中共分离到39株猪沙门菌,养猪场、屠宰场、销售市场所分离沙门菌检出率分别为51.28%,41.02%,7.70%;主要血清型为猪霍乱沙门菌、肠炎沙门菌、鼠伤寒型沙门菌,其阳性率分别为41.02%,38.46%,5.12%,未定型占15.38%;毒力因子共检出11种,其中检出率达到90%以上的和3种血清型共同携带的毒力因子结果一致有mogA、sseL、mgtC、bcfA、araB、stn,猪生产链3个环节共同携带的毒力因子有spvR、spvA、mogA、mgtC、araB、stn,其中养殖场毒力因子检出率最高,销售市场毒力因子检出率最低;猪霍乱沙门菌可引起小鼠的急性败血症,肠炎沙门菌和鼠伤寒沙门菌均可引起小鼠发病,但各器官的损伤则较轻。In order to understand the distribution characteristics and pathogenicity of the main virulence virulence factors of Salmonella typhimurium strains in Liaoning Province,a representative large-scale pig farm was selected from Liaoning based on the conserved genes of Salmonella genus,multiple PCR primers and serum agglutination test.Downstream pig slaughterhouses and meat markets collect samples of gut,feed and sewage,pork and other samples for Salmonella typhimurium and its serotype identification.Application of 14 major virulence factor-specific gene amplification assays for detection of Salmonella typhimurium to detect the distribution of virulence factors of different serotypes of Streptococcus suis,and using mouse challenge test and pathology techniques.The disease was observed and studied.The results showed that 39 strains of Salmonella were isolated from 412 samples collected from Liaoning.The detection rates of Salmonella isolated from pig farms,slaughterhouses and sales markets were 51.28%,41.02% and 7.70%,respectively.The main serotype was pig cholera.The positive rates of Salmonella,Salmonella enteritidis and Salmonella typhimurium were 41.02%,38.46% and 5.12%,respectively,and the undetermined type accounted for 15.38%.The results of virulence factor test showed that 11 virulence factors were detected,of which 6 species detected more than 90%,including mogA,sseL,mgtC,bcfA,araB,stn the same as the virulence of the three serotypes the factors.The virulence factors carried by the three links in the pig production chain include spvR,spvA,mogA,mgtC,araB,and stn,among which the detection rate of the virulence factor of the farm is the highest.Secondly,the higher detection rate is the slaughterhouse.The detection rate of virulence factors is the lowest in the sales market.Salmonella choleraesuis can cause acute sepsis in mice.Salmonella enteritidis and Salmonella typhimuriumcan cause disease in mice,but damage of various organs.It is lighter,and Se.nteritidis inoculation can cause intestinal enteritis in mice.

关 键 词：辽宁省 猪沙门菌 血清型 毒力因子 

分 类 号：S852.61[农业科学—基础兽医学]