肿瘤发生过程中髓源性抑制细胞能量代谢的特点  

The Energy Metabolism of Myeloid-derived Suppressor Cells in Tumor Development

作　　者：刘亚平 李鑫 郭子琦[1,2,3] 李晓玉 吴琼 周祖平[1,2,3] 杨程 LIU Ya-ping;LI Xin;GUO Zi-qi;LI Xiao-yu;WU Qiong;ZHOU Zu-ping;YANG Cheng(College of Life Science Guangxi Normal University,Guilin 541004,Guangxi,China;Research Center for Biomedical Sciences,Guangxi Normal University,Guilin 541004,Guangxi,China;Guangxi Universities Key Laboratory of Stem Cell and Pharmaceutical Biotechnology,Guilin 541004,Guangxi,China)

机构地区：[1]广西师范大学生命科学学院,中国广西桂林541004 [2]广西师范大学生物医学研究中心,中国广西桂林541004 [3]广西高校干细胞与医药生物技术重点实验室,中国广西桂林541004

出　　处：《生命科学研究》2019年第4期263-269,共7页Life Science Research

基　　金：广西自然科学基金资助项目(2018GXNSFAA138004);广西研究生教育创新计划项目(XYCSZ2018058,YCSW2018097)

摘　　要：髓源性抑制细胞(myeloid-derived suppressor cells, MDSCs)作为免疫调节细胞,在肿瘤发生和发展中起重要作用。糖代谢参与MDSCs 功能的调节,但是,对于肿瘤进程中MDSCs 代谢水平的变化,相关报道甚少。基于此,本研究利用小鼠肿瘤模型,采用流式细胞术先后分析肿瘤发生中MDSCs 的丰度、周期及线粒体质量,利用ELISA 试剂盒检测MDSCs 乙酰辅酶A 的含量,并在2-脱氧-D-葡萄糖(2-deoxy-D-glucose, 2-DG)改变糖代谢水平之后检测线粒体质量和细胞凋亡。结果发现:肿瘤发生中MDSCs 的丰度明显增加,进入分裂期的细胞数增多;肿瘤状态下MDSCs 乙酰辅酶A 的含量增加,线粒体质量显著增加;2-DG 处理后,肿瘤条件下MDSCs的线粒体质量恢复至正常水平且细胞凋亡减少。以上结果表明,在肿瘤发生过程中, MDSCs 主要依赖氧化磷酸化代谢获取能量,改变其糖代谢水平可能导致细胞功能变化。Myeloid-derived suppressor cells(MDSCs), as a class of immunoregulatory cells, play an important role in tumorigenesis and development. Glucose metabolism is involved in the regulation of MDSC function,but there are few reports on the changes of MDSC metabolic levels during tumor progression. Based on these considerations, the following experiments were performed using the mouse tumor model. The abundance, cell cycle and mitochondrial mass of MDSCs in tumor development were analyzed by flow cytometry, and the acetyl coenzyme A(ACA) content of MDSCs was detected by ELISA kit. Finally, mitochondrial mass and apoptosis were detected by flow cytometry after 2-deoxy-D-glucose(2-DG) changed the level of glucose metabolism.The results showed that the abundance of MDSCs increased significantly in tumorigenesis, and the number of cells entering the cell division phase increased. The content of ACA and the mitochondrial mass of MD-SCs increased significantly under the tumor conditions. Interestingly, after 2-DG treatment, the mitochon-drial mass of MDSCs returned to a normal level and apoptosis decreased under tumor conditions. The results indicated that MDSCs mainly depend on oxidative phosphorylation metabolism to acquire energy during tu-morigenesis, and that changes in glucose metabolism may lead to changes in cell functions.

关 键 词：髓源性抑制细胞(MDSCs) 肿瘤发生 氧化磷酸化 能量代谢 

分 类 号：Q28[生物学—细胞生物学]