Reconsideration on the effect of nitrogen on mixed culture polyhydroxyaIkanoate production toward high organic loading enrichment history  

作　　者：Zhiqiang Chen Lizhi Zhao Ye Ji Qinxue Wen Long Huang 

机构地区：[1]State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China [2]School of Civil Engineering,Lanzhou University of Technology,Lanzhou 730070,China

出　　处：《Frontiers of Environmental Science & Engineering》2019年第4期77-87,共11页环境科学与工程前沿（英文）

基　　金：the financial support of the National Key R&D Program of China (Grant No.2016YFC0401102-3);the National Natural Science Foundation of China (Grant No.51578183);the Nanqi Ren Studio, Academy of Environment&Ecology, Harbin Institute of Technology (No.HSCJ201703).

摘　　要：In most of the operating strategies for mixed microbial cultures polyhydroxyalkanoate (PHA) production, moderate organic loads and low nitrogen concentrations are used, however, the real waste streams contain variable concentrations of carbon and nitrogen. To evaluate the effect of enrichment history on PHA producer and production the various carbon and nitrogen levels were utilized during the accumulation phase. Different operating strategies were applied in three sequencing batch reactors (SBRs) subjected to aerobic dynamic feeding. The maximum PHA production of the enriched cultures under nutrient excess, limitation and starvation (Cmol/Nmol ratio of & 40 and oo, respectively) was evaluated in batch assays. A higher PHA content and biomass growth were achieved in the nutrients presence in comparison to the nutrient starvation condition. The cultures from the SBR treated under short sludge retention time, high organic loading rate, short cycle length (SBR#3) and nutrient excess reached the maximum PHA content (54.9%) and biomass increase (38.9%). Under nutrient limitation, the negative biomass growth was observed under nutrient starvation because of the sampling loss. The succession of microbial communities in SBRs and batch assays was analyzed using terminal restriction fragment length polymorphism. The SBR#3 had the best overall PHA production performance considering its high PHA content and productivity in all nutrient content, it indicates that nitrogen has a substantial impact on PHA yield especially when high organic loading rate enrichment history is involved.

关 键 词：POLYHYDROXYALKANOATE (PHA) Organic loading rate NITROGEN content Biomass growth ENRICHMENT HISTORY 

分 类 号：T[一般工业技术]