129株非结核分枝杆菌采用两种分子检测技术行菌种鉴定的结果分析  

Analysis on identification of 129 non-tuberculosis mycobacteria strains using two molecular biology techniques

作　　者：刘佳文[1] 吕红艳[1] 丁北川[2] 张治国[3] LIU Jia-wen;LYU Hong-yan;DING Bei-chuan;ZHANG Zhi-guo(Department of Clinical Laboratory,Beijing Geriatric Hospital,Beijing 100095,China)

机构地区：[1]北京老年医院检验科,100095 [2]北京结核病控制研究所中心实验室 [3]北京市昌平区结核病防治所

出　　处：《中国防痨杂志》2019年第9期999-1004,共6页Chinese Journal of Antituberculosis

摘　　要：目的分析北京地区3个单位非结核分枝杆菌(NTM)临床分离株采用芯片杂交法(简称“芯片法”)和16SrRNA基因测序(简称“基因测序”)进行菌种鉴定的结果,为临床分枝杆菌病的快速准确诊断提供科学依据。方法用芯片法和基因测序进行北京地区3个单位129株NTM临床分离株的菌种鉴定,实验操作者之间采用双盲法,对两种鉴定方法的结果进行比较和评价。结果129株NTM菌株中,有107株进行分枝杆菌基因测序,其中59株为胞内分枝杆菌(55.1%);29株为堪萨斯分枝杆菌(27.1%);5株为龟分枝杆菌/脓肿分枝杆菌(4.7%);3株为鸟分枝杆菌(2.8%);2株为偶发分枝杆菌(1.9%);2株为戈登分枝杆菌(1.9%);1株为蟾蜍分枝杆菌(0.9%);1株为草分枝杆菌(0.9%);1株为瘰疬分枝杆菌(0.9%);4株为其他NTM(3.7%)。有116株进行芯片法分枝杆菌菌种鉴定,且有96株同时进行芯片法和基因测序法检测,两种方法进行菌种鉴定的符合率为88.5%(85/96)。基因测序发现有3株外来菌株,分别为明尼苏达分枝杆菌、熊本分枝杆菌、提门分枝杆菌(M.temen)。结论北京3家机构NTM菌种以胞内分枝杆菌、堪萨斯分枝杆菌和龟分枝杆菌/脓肿分枝杆菌为主。用芯片法和基因测序对NTM临床分离株进行菌种鉴定的一致率较高。Objective To analyze the results of strains’ identification of clinical isolates of non-tuberculosis mycobacteria (NTM) from three tuberculosis institution in Beijing using chip hybrization (chip method) and 16S rRNA gene sequencing (gene sequencing), and to provided scientific evidences for rapid and accurate diagnosis of clinical mycobacterial disease.Methods One hundred and twenty-nine strains of NTM from 3 institutes in Beijing were performed to identify using chip method and gene sequencing which the blind method was conducted among technicians. The results of two identifications were compared and evaluated.Results Of the 129 strains of NTM, 107 strains were performed by gene sequencing, the identification results were as follows: M.intracellulare in 59 strains (55.1%), M.kansasii in 29 strains (27.1%), M.chelonae/abscessus in 5 strains (4.7%), M.avium in 3 strains (2.8%), M.fortuitum and M.gordonae in each 2 strains (1.9% each), M.xenopi, M.phlei and M.scrofulaceum in each one strain (0.9% each) and other NTM in 4 strains (3.7%). Of 116 strains performed by chip method, 96 strains were performed with both methods. The coincidence rate of the two methods was 88.5%(85/96). There were three foreign strains of NTM identified by gene sequencing, which were M.minnesotense, M.kumamotonense and M.temen.Conclusion The main strains of NTM from three institutes in Beijing are M.intracellulare, M.kansasii and M.chelonae/abscessus. The consistency rate of identification of clinical isolates of NTM by chip method and gene sequencing is high.

关 键 词：分枝杆菌 非典型性 生物学鉴定法 芯片分析技术 分子探针技术 寡核苷酸序列分析 结果与过程评价(卫生保健) 

分 类 号：R378.91[医药卫生—病原生物学]