文冠果种仁对SD大鼠的致畸作用  

Teratogenic effects of Xanthoceras sorbifolia Bunge seed on SD rats

作　　者：李慧[1] 李伟[2] 温成丽 唐慧[1] 陈萌 周雯[1] 张天亮[1] 张静[1] 范轶欧[1] 程东[1] Li Hui;Li Wei;Wen Chengli;Tang Hui;Chen Meng;Zhou Wen;Zhang Tianliang;Zhang Jing;Fan Yiou;Cheng Dong(Department of Toxicology,Shandong Provential Center for Disease Prevention and Control,Jinan 250014,China;Huaiyin District Center for Disease Prevention and Control,Jinan 250022,China)

机构地区：[1]山东省疾病预防控制中心毒理与功能检验所,济南250014 [2]山东省济南市槐荫区疾病预防控制中心,济南250022

出　　处：《卫生研究》2019年第5期799-802,806,共5页Journal of Hygiene Research

基　　金：国家自然科学基金青年科学基金(No.NFSC31101068/C120206)

摘　　要：目的研究文冠果种仁对SD大鼠的致畸作用。方法于SPF级SD大鼠孕期第6~15天,经口灌胃给予孕鼠文冠果种仁2. 0、4. 0和8. 0 g/kg,并于分娩前1天处死母鼠,剖检检查母鼠受孕和仔鼠发育情况。对骨骼行茜素红染色并进行检查,对内脏进行Bouins液固定并检查。结果文冠果种仁各剂量组的母体体重、体重增重、子宫连胎重、体重净增重,着床数、黄体数、吸收胎数、活胎数、死胎数及百分率,胎鼠外观、骨骼、内脏的单项畸胎率和总畸胎率,以及有畸形胎仔的窝百分率等,与阴性对照组比较差异无统计学意义(P>0. 05)。结论在该实验条件下,文冠果种仁对妊娠SD大鼠无母体毒性,对胎鼠无致畸性和胚胎发育毒性,其母体毒性的未观察到有害作用剂量(NOAEL)和胎鼠的最小致畸剂量>8. 0 g/kg。OBJECTIVE To study the teratogenic effect caused by Xanthoceras sorbifolia Bunge seed on SD rats. METHODS The experiments were performed in the groups of 2. 0, 4. 0 and 8. 0 g/kg, purified water negative control group and cyclophosphamide positive control group. On the 6 th to 15 th day of pregnancy,the SPF SD rats were exposed to Xanthoceras sorbifolia Bunge seed. All the rats were sacrificed on the day before delivery. Examination were performed on the bones stained by alizarin red and internal organs fixed with Bouins fluid. RESULTS Maternal body weight,weight gain,uterine fetal weight,net weigh,bed number,corpus luteum number,absorbing births number,live births number,still birth number and percentage and the abnormal rate of appearance,bone,internal organs of each dose group of Xanthoceras sorbifolia,there was no statistical significant difference between Bunge seed groups and negative control group.CONCLUSION Under the conditions of this experiment,the Xanthoceras sorbifolia Bunge seed had no maternal toxicity to pregnant SD rats,no teratogenic and developmental toxicity to fetal rats. No Observed Adverse Effect Level of maternal toxicity and the minimum teratogenic dose of fetal rats is >8. 0 g/kg.

关 键 词：文冠果 致畸作用 母体毒性 发育毒性 

分 类 号：R132.7[医药卫生—劳动卫生] Q949.754[医药卫生—公共卫生与预防医学]