贵州仡佬族和苗族人群24个常染色体STR基因座的遗传多态性及遗传关系分析  

Genetic polymorphisms and genetic relationship of 24 autosomal STR loci in Gelao and Miao population of Guizhou

作　　者：刘亚举 李瑾 岳俊涛 李学博[2] 石美森 LIU Ya-ju;LI Jin;YUE Jun-tao;LI Xue-bo;SHI Mei-Sen(Institute of Criminal Science and Technology,Henan Province Xuchang Public Security Bureau,Xuchang,Henan 461000,China;Key Laboratory of Evidence Identification in Universities of Shandong Province,Shandong University of Political Science and Law,Jinan 250014,China;Institute of Investigation,Criminal Justice College of China University of Political Science and Law,Beijing 100088,China)

机构地区：[1]河南许昌市公安局刑事科学技术研究所,河南许昌461000 [2]山东省高校证据鉴识重点实验室,山东政法学院,济南250014 [3]中国政法大学刑事司法学院侦查学研究所,北京100088

出　　处：《解放军医学杂志》2019年第8期682-689,共8页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(81671874);中国博士后科学基金(2017M612701);山东省自然科学基金(ZR2014HQ018)~~

摘　　要：目的调查贵州仡佬族和苗族人群24个常染色体短串联重复序列(STR)基因座的遗传多态性,探讨其群体遗传关系。方法应用SureID■PanGlobal试剂盒对贵州399名仡佬族和333名苗族无关个体进行DNA扩增,采用3500XL遗传分析仪进行电泳分析,GeneMapperID-Xv1.5软件分析等位基因片段大小。统计分析24个STR基因座的频率数据和法医遗传学参数,并与其他地区已有人群数据进行比较。结果贵州仡佬族和苗族各基因座个体识别率(DP)值分别为0.7833~0.9909和0.8010~0.9909,多态信息含量(PIC)值分别为0.5608~0.9385和0.5677~0.9414。累积个体识别率(TDP)分别为1-7.6036×10^-30和1-6.8630×10^-30,累积非父排除率(CPE)分别为1-1.9608×10^-11和1-1.9738×10^-11。Nei'sDA遗传距离矩阵分析发现,贵州仡佬族与湖北汉族遗传距离最小(0.0205),与云南苗族的遗传距离最大(0.0449);贵州苗族与湖南汉族的遗传距离最小(0.0033),与云南苗族的遗传距离最大(0.0363)。结论24个STR基因座在贵州仡佬族和苗族人群中具有丰富的遗传多态性。研究不同民族群体的遗传多样性对了解其起源、迁移以及相互关系有重要意义。Objective To investigate the genetic polymorphisms of 24 autosomal short tandem repeats (STR) loci in Gelao and Miao populations dwelled in Guizhou province,and explore the population genetic relationships and evaluate their application value on forensic medicine.Methods The DNA samples of 732 unrelated individuals (399 Guizhou Gelao population and 333 Guizhou Miao population) were amplified using SureID? PanGlobal kit,and the PCR products were analyzed by electrophoresis through 3500XL genetic analyzer.The fragment sizes of alleles were subsequently analyzed by GeneMapper ID-X v1.5.Allele frequencies and forensic genetic parameters of 24 STR loci were statistically analyzed and compared with the available data of other populations from different races and regions.Results For Guizhou Gelao and Miao populations,the individual discrimination power (DP) ranged from 0.7833 to 0.9909 and 0.8010 to 0.9909,respectively;the polymorphic information content (PIC) ranged from 0.5608 to 0.9385 and 0.5677 to 0.9414,respectively;the total discrimination power (TDP) were 1-7.6036×10^-30 and 1-6.8630×10^-30,respectively,and the cumulative probability of exclusion (CPE) were 1-1.9608×10^-11 and 1-1.9738×10^-11, respectively.Analysis with the matrix of Nei's DA genetic distance indicated that the genetic distance was the smallest (0.0205) between Guizhou Gelao and Hubei Han populations,while was the largest (0.0449) between Guizhou Gelao and Yunnan Miao populations;the genetic distance was minimum (0.0033) between Guizhou Miao and Hunan Han populations,while was maximal (0.0363) between Guizhou Miao and Yunnan Miao.Conclusions The 24 STR loci are abundant in genetic polymorphism in Guizhou Gelao and Miao populations.It is of great significance to study the genetic diversity of different ethnic groups in order to understand their origin,migration and interrelationship.

关 键 词：常染色体短串联重复序列 遗传多态性 Neighbor-Joining系统发育树 遗传关系 

分 类 号：R349.69[医药卫生—基础医学]