野桑蚕scalloped同源基因的克隆和序列分析  

Cloning and sequence analysis of scalloped homologous gene of wild silkworm

作　　者：朱文东 ZHU Wen-dong(College of Modern Agriculture and Biotechnology,Ankang University/Shaanxi Key Laboratory of Sericulture,Ankang 725000,Shaanxi,China)

机构地区：[1]安康学院现代农业与生物科技学院/陕西省蚕桑重点实验室

出　　处：《湖北农业科学》2019年第17期123-128,共6页Hubei Agricultural Sciences

基　　金：陕西省教育厅重点实验室科研计划项目（18JS002;16JS001）;陕南秦巴山区生物资源综合开发协同创新中心项目（QBXT-Z(Z)-15-5）;安康学院硕士点培育学科建设专项(2016AYXNZX018)

摘　　要：Hippo通路与昆虫的生长、发育和变态等过程密切相关,在动物不同物种之间高度保守.scalloped基因是Hippo通路元件Yki的偶联因子之一,参与调控基因的表达.克隆了野桑蚕(Bombyx mandarina)scalloped基因的完整开放阅读框(ORF)及其上下游的部分非编码序列.序列联配分析表明,与家蚕相比,野桑蚕scalloped基因序列存在多处的核酸片段缺失、插入及位点差异.结构域分析表明,野桑蚕Scalloped蛋白与家蚕、黑腹果蝇、小鼠和人的蛋白结构类似,均含有完整的TEAD蛋白家族典型TEA结构域和PDB结构域,但野桑蚕编码蛋白缺失蛋白C末端序列.系统发生树分析表明,昆虫Scalloped蛋白与脊椎动物TEF3可能存在共同的起源;家蚕Scalloped在进化中出现晚于野桑蚕且经历较多遗传选择过程.Hippo pathway is closely related to the physiological and biochemical processes of insect growth,metamorphosis and development,and highly conserved among different species.Scalloped is one of terminal action factor of Hippo pathway,which regulates the expression of a large number of genes.In this study,the complete ORF and partial UTR sequence of scalloped in wild silkworm (Bombyx mandarina) were cloned.Indels and variable sites were discovered after sequences analysis between Bombyx mori and Bombyx mandarina.The domain structure analysis shown that the encoding protein of scalloped in wild silkworm,domestic silkworm,fruit fly,mice and human have the similar domain architecture,while partial of the C-terminal of wild silkworm scalloped protein absent.The phylogenetic analysis showed that the Scalloped in insects and TEF3 in vertebrate could have a common origin,and the Scalloped of domestic silkworm could be appeared later than that in wild silkworm,and experienced more genetic selection process.

关 键 词：野桑蚕(Bombyx mandarina) Hippo通路 scalloped基因 

分 类 号：Q785[生物学—分子生物学]