一个植物原纤维蛋白基因的克隆及其表达特性分析  

Cloning and expression analysis of a fibrillin gene in plant

作　　者：姜瑶瑶 李静[2] 蔡年俊 陈剑平 张恒木[2] JIANG Yaoyao;LI Jing;CAI Nianjun;CHEN Jianping;ZHANG Hengmu(College of Forestry and Biotechnology,Zhejiang A&F University,Lin an311300,China;Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou310021,China;Institute of Plant Virology,Ningbo University,Ningbo315211,China)

机构地区：[1]浙江农林大学林业与生物技术学院,浙江杭州311300 [2]浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021 [3]宁波大学植物病毒学研究所,浙江宁波315211

出　　处：《浙江农业学报》2019年第9期1399-1404,共6页Acta Agriculturae Zhejiangensis

基　　金：浙江省自然科学基金(LQ14-C140003)

摘　　要：植物原纤维蛋白(fibrillin, FBN)作为一大类保守的蛋白,广泛分布于植物界,但其在本氏烟( Nicotiana benthamiana )中的生物学特性和功能迄今尚不清楚。为了分析其表达特性和功能,采用RT-PCR技术从本氏烟中扩增并克隆了1个 FBN 基因( NbFBN )。进化树分析显示, NbFBN 和拟南芥 FBN1a 、 FBN1b 的亲缘关系较近;同源分析表明,它与不同植物来源的 FBN 基因高度同源,其C-端部分尤为保守。定量分析发现, NbPAP 在叶片和花中的表达水平较高,同时发现该基因受到干旱胁迫和激素ABA的诱导,表明该基因可能参与非生物逆境响应过程。Plant fibrillin (FBN),a large family of conserved proteins,is widely distributed in plant kingdom,but its biological properties and functions in Nicotiana benthamiana are still unclear. To analyze its expression and function,an FBN gene ( NbFBN ) was amplified and cloned from Nicotiana benthamiana by RT-PCR. Phylogenetic tree analysis showed that the gene was closely related to FBN1a and FBN1b in Arabidopsis thaliana . Homology analysis indicated that it was highly homologous to different FBN genes and its C-terminal part was particularly conserved in several kinds of plants. Quantitative analysis showed that NbFBN was highly expressed in leaves and flowers of Nicotiana benthamiana, and that the gene was found to be induced by drought stress and hormone ABA,indicating that the gene might be involved in response to the abiotic stresses.

关 键 词：植物原纤维蛋白 同源性分析 定量PCR 非生物逆境 

分 类 号：Q943.2[生物学—植物学]