褪黑素拮抗高糖导致的小鼠成骨细胞凋亡作用研究  

Effect of melatonin on apoptosis of mouse osteoblasts induced by high glucose

作　　者：刘司琪[1] 梁单 李冰[1] 郭琳[2] Liu Siqi;Liang Dan;Li Bing;Guo Lin(Troops of 95988 unit, Changchun 130000, China;Troops of 93253 unit,Dalian 110000,China)

机构地区：[1]中国人民解放军95988部队,吉林长春130000 [2]中国人民解放军93253部队,辽宁大连110000

出　　处：《甘肃医药》2019年第6期481-484,共4页Gansu Medical Journal

摘　　要：目的:探讨褪黑素对高糖诱导成骨细胞凋亡的保护作用及作用机理。方法:小鼠成骨细胞株(MC3T3-E1)贴壁培养后,分成三组:对照组,高糖组,高糖+褪黑素组。采用MTT方法检测小鼠成骨细胞增殖情况,采用TUNEL、Western-blot、流式细胞术等方法检测MC3T3-E1凋亡,采用Western-blot测定PI3K/AKT通路相关蛋白水平。结果:高糖可以抑制成骨细胞增殖,高糖作用24、48h的半数抑制浓度分别是49.3mmol/L和36.7mmol/L。高糖诱导细胞凋亡增加。40mmol/L高糖作用细胞48h,细胞的凋亡达到了40.8%,凋亡相关蛋白表达也增加。而褪黑素可以激活PI3K/AKT保护通路,明显拮抗高糖诱导的成骨细胞凋亡。30μmol/L褪黑素可以使高糖(40mmol/L)诱导的凋亡率从40.8%降低到28.3%。凋亡相关蛋白表达减低。结论:褪黑素通过上调PI3K/AKT通路表达,拮抗高糖诱导的成骨细胞的凋亡。Objective:To investigate the role of melatonin in inhibiting high glucose induced apoptosis in osteoblasts. Methods: The cell proliferation rate was detected by MTT assay. The apoptosis was detected by flow cytometry, TUNEL and western-blot. The PI3K / AKT pathway protein expression was detected by western-blot. Results:High glucose could inhibit the proliferation of osteoblasts, and the 24h-IC50 and 48h-IC50 were 49.3 mmol/L and 36.7 mmol/L.High glucose could induce cell apoptosis. After treatment of 40 mmol/L high glucose for 48 hours, the apoptosis of the cells reached to 40.8%, and the expression of apoptosis-related proteins were upregulated.Melatonin could activate the PI3K/AKT protective pathway and antagonize high glucose-induced osteoblast apoptosis.30μmol/L melatonin could reduced the high glucose(40 mmol/L)-induced apoptosis rate from 40.8% to 28.3%. The expression of apoptosis-related proteins was als downregulated.Conclusion:Melatonin can effectively inhibit the apoptosis of osteoblasts induced by high glucose through activating the PI3K / AKT pathway.

关 键 词：高糖 褪黑素 细胞凋亡 小鼠成骨细胞 

分 类 号：R587.1[医药卫生—内分泌]