龙脑樟Actin基因的克隆与序列分析  

Cloning and Sequence Analysis of the Actin Gene from the Cirmamonun camphora

作　　者：张君诚 许晓颖 邹函卓 杨琳 ZHANG Jun-cheng;XU Xiao-ying;ZOU Hang-zhuo;YANG Lin(Engineering Research Center of Medicinal plant Exploration and Utilization in Colleges and Universities of Fujian Province,Sanming 365004,China;Forestry College,Fujian Agriculture and Forestry University,Fuzhou 350001,China)

机构地区：[1]天然药物开发利用福建省高校工程研究中心,福建三明365004 [2]福建农林大学林学院,福建福州350001

出　　处：《三明学院学报》2019年第4期13-16,共4页Journal of Sanming University

基　　金：福建省高校工程研究中心开放课题(ZD1602)

摘　　要：利用同源克隆的方法设计特异性引物,从龙脑樟中获得一个333bp的基因片段,编码110个氨基酸。该片段的蛋白分子量为12.37ku,理论等电点为5.03,平均疏水性(GRAVY)为-0.312,α螺旋、β片层和无规则卷曲的比例分别为40.04%、10.09%和45.87%。同源比对和进化树分析结果都表明,龙脑樟Actin基因与香樟、山鸡椒的Actin基因具有较高的同源性。龙脑樟Actin基因片段的克隆,为深入研究该基因的表达调控及龙脑樟遗传与进化关系提供理论基础。A 333 bp gene fragment encoding 110 amino acids was obtained from Cirmamonun camphora by homologous cloning.The molecular weight was 12.37 ku,the theoretical pI was 5.03,and the grand average of hydropathicity (GRAVY) was -0.312.Moreover,the ratios of α helices,extended strand and random coil were 40.04%,10.09% and 45.87%,respectively.The results of homology comparison and phylogenetic tree analysis showed that the Actin gene of Cirmamonun camphora had high homology with Cirmamonun camphora and Capsicum capsicum.Cloning the Actin gene from Cirmamonun camphora was the basis for further study of the gene expression and evolutionary relationship.

关 键 词：龙脑樟 ACTIN基因 克隆 序列分析 

分 类 号：Q943.2[生物学—植物学]