不同时长UVA照射对黄瓜霜霉病发生和生理特征的影响  

Effects of Different Duration of UVA Irradiation on the Occurrence and Morphological Characteristics of Cucumber Downy Mildew

作　　者：高亚轻 苑磊 种道齐 黄晓梅 刘世琦[1] GAO Yaqing;YUAN Lei;CHONG Daoqi;HUANG Xiaomei;LIU Shiqi(State Key Laboratory of Crop Biology,Key Laboratory of Horticultural Crop Biology,Ministry of Agriculture,College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018)

机构地区：[1]山东农业大学园艺科学与工程学院作物生物学国家重点实验室农业部园艺作物生物学重点开放实验室

出　　处：《北方园艺》2019年第17期1-6,共6页Northern Horticulture

基　　金：国家公益性行业(农业)科研专项资助项目(200903018)

摘　　要：以“津优35号”黄瓜为试材,在日光温室中采用不连续照射方式对黄瓜植株进行夜间辐照处理,UVA照射光强为60μW·cm^-2,照射时间分别为0、3、4、5 h·d^-1,以不辐照为对照,以期探明不同照射时间对黄瓜霜霉病发生及植株生长的影响。结果表明:随着UVA照射时间增加,促进黄瓜生长及抗霜霉病的作用更加明显。UVA照射20 d后防效高达85.92%。随着UVA照射时间延长光合色素含量整体上均增加;UVA照射5 h·d^-1的处理,株高、茎粗、叶面积分别比CK高出36.05%,42.17%,47.01%。UVA照射5 h·d^-1处理的果实横径、单果质量、果形指数分别比CK高出94.44%、84.21%、18.60%。在5 h·d^-1的紫外光UVA辐射下,处理14 d和21 d后,过氧化物酶(POD)活性均显著高出CK 80.53%、89.12%。UVA辐射5 h·d^-1,处理21 d后,苯丙氨酸解氨酶(PAL)活性显著高于0、3、4 h分别是4.64 U·g^-1·min^-1>2.48 U·g^-1·min^-1>3.68 U·g^-1·min^-1>4.35 U·g^-1·min^-1。夜间辐照5 h·d^-1下,处理7、14、21 d时类黄酮含量与CK相比均有显著的差异分别比CK高出20.77%、67.42%、89.37%。‘Jinyou 35’ cucumber was used as test material in a solar greenhouse.The cucumber plants were treated with nighttime irradiation,and no light was added as a control.The test used a discontinuous irradiation method,and the UVA irradiation intensity was 60 μW·cm^-2,and the irradiation time was 0,3,4,5 h·d^-1,respectively.In order to find out the effects of different irradiation time on the occurrence of cucumber downy mildew and plant growth.The results showed that with the increase of UVA irradiation time,the effect of promoting cucumber growth and resistance to downy mildew was more obvious.After 20 days of UVA irradiation,the control effect was as high as 85.92%.The photosynthetic pigment content increased with the increase of UVA irradiation time.The plant height,stem diameter and leaf area were 36.05%,42.17%,47.01% higher than CK after UVA irradiation for 5 h·d^-1.The cross-stalk,single fruit quality and fruit shape index of the fruits treated by UVA irradiation were 94.44%,84.21%,18.60% higher than those of CK,respectively.Under UVA radiation of 5 h·d^-1.POD activity was significantly higher than that of CK after 14 days and 21 days,respectively,which was 80.53% and 89.12%.UVA radiation was 5 h·d^-1.After 21 days treatment,the PAL activity was significantly higher than 0,3,4 h·d^-1,which was 4.64 U·g^-1·min^-1>2.48 U·g^-1·min^-1>3.68 U·g^-1·min^-1>4.35 U·g^-1·min^-1.At night exposure at 5 h·d^-1,the flavonoid content was significantly different from CK at 7,14,21 days,respectively,20.77%,67.42%,89.37% higher than CK.

关 键 词：紫外光 UVA 抗氧化酶活性 黄瓜 霜霉病 

分 类 号：S642.2[农业科学—蔬菜学]